Exogenous antizyme 1 gene transfection inhibits proliferation and promotes apoptosis of human neuroblastoma SH-SY5Y cells in vitro / 南方医科大学学报

<p><b>OBJECTIVE</b>To study the effect of antizyme 1 (ZA1) gene transfection on the cell proliferation, cell cycle and apoptosis of human neuroblastoma SH-SY5Y cells in vitro.</p><p><b>METHODS</b>The recombinant eukaryotic expression vector pAZ1m was constructed by cloning mutant AZ1 gene into the vector pEGFP-N1, and subsequently transfected in SH-SY5Y cells. The transfected cell proliferation was examined using MTT assay, and the changes in cell cycle and apoptosis were assayed using flow cytometry analysis. RT-PCR was performed to measure cyclin D1 and caspase-3 mRNA expressions, Western blotting carried out to examine cyclin D1 protein expression, and the changes in caspase-3 activity were detected using a caspase-3 detection kit.</p><p><b>RESULTS</b>AZ1 gene transfection significantly inhibited the proliferation of SH-SY5Y cells, causing cell cycle arrest at G0/G1 stage and down-regulated cyclin D1 and up-regulated caspase-3 expressions. Obviously increased caspase-3 activity was also observed in the transfected cells.</p><p><b>CONCLUSION</b>Exogenous AZ1 gene transfection can inhibit the proliferation and cause cell cycle arrest of SH-SY5Y cells by down-regulating cyclin D1 expression. Up-regulated caspase-3 expression resulting from AZ1 gene transfection may induce apoptosis of the neuroblastoma cells.</p>

Oligonucleotide Array Comparative Genomic Hybridization and Its Application / 中国生物工程杂志

The array CGH technique (Array Comparative Genome Hybridization) has been developed to detect chromosomal copy number changes on a genome-wide and/or high-resolution scale. It is mainly used in human genetics and oncology. Generally PCR amplified bacterial artificial chromosomes (BACs) or cDNAs have been spotted on the arrays as probes. Recently, however, oligonucleotide arrays designed with more flexibility and provide much higher resolution with high sensitivity, have been successfully explored in stead of BAC array CGH and can save considerable time and efforts. There will be a gradual transition from BAC array CGH to oligonucleotide array CGH in the coming years. The combination of oaCGH and other high-through put analysis can lead to discoveries of a host of novel oncogenes, tumor suppressors as well as tumor drug resistance genes. Some major platforms of oaCGH concerning their spatial resolution, optimal probe length, sensitivity, specificity and application in recent years were compared.