Secondary metabolites of endophytic fungus Aspergillus sp. J218 from Anectochilus roxburhii / 药学实践杂志

Objective To isolate the secondary metabolites of endophytic fungus Aspergillus sp. J218 from Anectochilus roxburhii. Methods Different chromatographic methods, including Sephadex LH-20 and silica gel chromatography as well as HPLC, were used to isolate compounds from the EtOAc fraction of the solid fermentation of J218, and their structures were identified by spectral methods. Results Ten compounds were isolated from the fermentation of J218 and their structures were individually identified as kotanin（1）, flavasperone（2）, aurasperone B（3）, fonsecinone B（4）, fonsecinone D（5）, ensidol A（6）, fonsecinone A（7）, fonsecinone C（8）, aurasperone A（9）, and fonsecinone F（10）. Conclusion Most compounds isolated from endophytic fungus Aspergillus sp. J218 in Anectochilus roxburhii were identified as dimeric naphthopyrones. These results suggest that this strain contains rich dimerization synthase, which could provide clues for the further exploration of the rational biosynthesis pathway of dimeric naphthopyrones in this strain.

Capecitabine causes myocardial damage in gastric cancer chemotherapy and its early prediction / 中国医师进修杂志

Objective:To evaluate the effect of serum cardiac troponin I (cTnI) and B-type brain natriuretic peptide (BNP) on early prediction of myocardial damage in gastric cancer patients treated with capecitabine chemotherapy.Methods:From July 2017 to June 2018, 88 patients with advanced gastric cancer who received treatment in Liaoning Cancer Hospital & Cancer Hospital of China Medical University were selected for retrospective analysis. According to different treatment methods, they were divided into paclitaxel combined with cisplatin (PC) treatment group and PC combined with capecitabine(PCX) treatment group. Serum cTnI and BNP were used as indexes before treatment, after treatment and three months after treatment. At the same time, the changes of ECG before and after treatment were analyzed.Results:At the end of the course of treatment, the serum cTnI concentration of PCX treatment group and PC treatment group was (0.582 9 ± 0.012 4) μg/L and (0.059 8 ± 0.011 2) μg/L, respectively, with statistical significance ( P<0.05); three months after the course of treatment, the serum cTnI concentration of the two groups was (0.558 5 ± 0.015 6) μg/L and (0.051 8 ± 0.009 9) μg/L respectively, with statistical significance ( P<0.05). And at the end of the course, the serum BNP concentration of PCX treatment group and PC treatment group was (495.6 ± 35.6) ng/L and (154.3 ± 29.8) ng/L respectively, with statistical difference between the two groups ( P<0.05). Three months after chemotherapy, the serum BNP concentration of the two groups was (155.5 ± 29.6) ng/L and (139.6 ± 30.1) ng/L, with no statistical difference ( P>0.05). There was no significant difference of BNP concentration between the two groups after treatment for 3 months ( P>0.05). The average abnormal rate of ECG before treatment was 21.6% (9.5 cases on average) and after treatment was 28.4% (12.5 cases on average), there was no significant difference between the two groups ( P>0.05). Conclusions:Early detection of cTnI and BNP concentrations can predict early myocardial damage in gastric cancer patients receiving capecitabine chemotherapy, and timely adjustment of the regimen can make the clinical application of capecitabine safer and more effective.

4-OHT inhibits cell migration by reversal of eMT in triple negative breast cancer cells / 中国医师进修杂志

Objective To investigate whether 4-OHT could decrease the ability of invasion and metastasis by reversal of EMT in TNBC. Methods Inverted microscope was used for observing cell morphological change. Cell migration ability was measured using transwell assay. Expression of E-cadherin, Vimentin and Actin proteins were analyzed by Western blot. The distribution of E-cadherin and Vimentin were analyzed by immune- fluorescence microscopy. Relative levels of miR-200c were measured by Real-time PCR. Transient transfection was performed using Lipofectamine 2000 reagent. Animal model was established to test the migration rate of lung metastasis and metastase focals. The HE staining was performed in mice lung tissue using Immunohistochemistry to confirm the metastasis. Results 4-OHT upregulates E-cadherin expression, downregulates vimentin expression to reverse EMT in TNBC cells. 4-OHT decreases migration ability of MDA-MB-231 and MCF-7/ADR cells by transwell test to 59.4% and 43.2% ; The migration ability of cells decrease to 66.7% . 4-OHT up-regulates the expression of miR-200c in MDA-MB-231 and MCF-7/ADR cells to 280% and 450%. Inhibits expression of miR-200c, 4-OHT cannot decrease migration ability of TNBC cells. Conclusions 4-OHT could decrease the ability of invasion and metastasis, up-regulate miR-200c and reverse EMT in TNBC.

Expression of phytase gene phyA in Yarrowia lipolytica po1h / 生物工程学报

Using the polymmerse chain reaction (PCR), we amplified the phytase gene phyA from Pichia pastoris GS115-phyA in Aspergillus niger NRRL3135 without the signal peptide sequence and intron sequence,. Then, it was cloned into pINA1297 vector to generate a recombinant vector of pINA1297-phyA. pINA1297-phyA was linearized and transformed into Yarrowia lipolytica po1h by the lithium acetate method. The positive transformants were obtained by YNB(casa) and PPB plates, after induced in YM medium at 28 degrees C for 6 day. The activity of the expressed phytase phyA reached 636.23 U/mL. The molecular weight of the enzyme was 130 kDa measured with SDS-PAGE analysis, whereas its molecular size reduced to 51 kDa after deglycosylation which is correspond with theoretical value. The enzymatic analysis of the recombinant phytase phyA revealed its optimal pH and temperature was 5.5 and 55 degrees C, which had high activity after incubated in pH ranged from 2.0 to 8.0 for 1 h. Moreover, its activity remained 86.08% after exposure to 90 degrees C for 10 min. It also was resistant to pepsin or trypsin treatment.