Detection of chromosomal translocations of DUSP22 and TP63 in ALK-negative anaplastic large cell lymphoma by fluorescence in situ hybridization and related clinical relevance / 中华病理学杂志

Objective@#To correlate chromosomal translocations of DUSP22 or TP63 with clinical significance in ALK-negative anaplastic large cell lymphoma (ALK-ALCL).@*Methods@#Thirty-two patients with ALK-ALCL were selected from January 2004 to January 2014 at Fujian Provincial Hospital for the detection of chromosomal translocations of DUSP22 and TP63 by fluorescence in situ hybridization (FISH). The relationship between DUSP22 and TP63 chromosomal translocations and the clinicopathological parameters of ALK-ALCL was analyzed.@*Results@#Among the 32 ALK-ALCL patients, 7(21.8%) had DUSP22 gene rearrangement (DUSP22+ALK-ALCL). Three patients (9.4%) had TP63 gene rearrangement (TP63+ ALK-ALCL). There were 22 patients (68.8%) without rearrangement of either DUSP22 or TP63 (DUSP22-TP63-ALK-ALCL). The patients with DUSP22+ ALK-ALCL were among the younger, and the patients with TP63+ ALK-ALCL were among the elder. The mean age of patients with DUSP22-TP63-ALK- ALCL was between those of DUSP22+ALK-ALCL and TP63+ ALK-ALCL (P<0.05). Based on Ann Arbor staging, incidence of DUSP22 gene rearrangement decreased as the clinical stage of ALK-ALCL increased (P<0.05). Incidence of TP63 gene rearrangement cases increases in patients at more advanced clinical stage(P<0.05). The five-year survival rate and prognosis of patients with DUSP22+ALK-ALCL were the highest. Patients with TP63+ ALK-ALCL had the lower five-year survival and the worse prognosis (P<0.05).@*Conclusion@#Presences of DUSP22 and TP63 chromosomal translocations correlate with the clinical stages and prognosis of ALK-ALCL and may be used for the differential diagnosis, determination of tumor aggressiveness and prognostication of ALK-ALCL.

Expression of microRNA in ALK-negative anaplastic large cell lymphoma and CD30-positive peripheral T cell lymphoma, not otherwise specified / 中华病理学杂志

<p><b>OBJECTIVE</b>To study the role of microRNAs (miRNAs) in ALK-negative anaplastic large cell lymphoma and CD30 positive peripheral T cell lymphoma (not otherwise specified), and discuss the pathogenesis of miRNAs in ALK-negative anaplastic large cell lymphoma.</p><p><b>METHODS</b>Three cases of ALK-negative anaplastic large cell lymphoma of lymph node, 3 cases of CD30-positive peripheral T cell lymphoma (not otherwise specified) of lymph node and 3 cases of reactive hyperplasia of lymph node were detected by high flow microarray of miRNAs. The method of real-time quantitative polymerase chain reaction was further applied for 7 miRNAs in 15 cases of ALK-negatie anaplastic large cell lymphomas of lymph node and 15 cases of CD30-positive peripheral T cell lymphoma (not otherwise specified) of lymph node.</p><p><b>RESULTS</b>The significant difference of 13 miRNAs was found between ALK-negative anaplastic large cell lymphoma and CD30 positive peripheral T cell lymphoma (not otherwise specified) (P < 0.05), of which the result of 5 miRNAs was consistent with miRNAs expression spectrum: miR-664b-5p, miR-1275, miR-4739, miR-4736 and miR-504-5p, the difference was statistically significant (P < 0.05). Compared with reactive hyperplasia of lymph nodes, miR-664b-5p, miR-1275 and miR-4739 were significantly under-expressed (P = 0.004, P = 0.021, P = 0.031) and miR-4736 and miR-504-5p were significantly over-expressed (P = 0.009, P = 0.007) in ALK negative anaplastic large cell lymphoma.</p><p><b>CONCLUSIONS</b>MiR-664b-5p, miR-1275, miR-4739, miR-4736 and miR-504-5p may become an important indicator in the differentiation ALK-negative anaplastic large cell lymphoma from CD30-positive peripheral T cell lymphoma (not otherwise specified). MiR-4739, miR-4736 and miR-1275 may play important role in pathogenesis of negative-anaplastic large cell lymphoma by target genes: TNFRSF8 and TMOD1.</p>

Primary study of loss of heterozygosity on chromosome 1p36 in invasive ductal breast carcinoma / 肿瘤研究与临床

Objective The study of loss of heterozygosity (LOH) on chromosome 1p36 was performed to locate the deletion areas probably harboring tumor suppressor genes in invasive ductal breast carcinoma not otherwise specified (IDC NOS).Methods Eighty paired breast cancer/normal tissue DNA samples were examined for LOH on chromosome lp36 using eight polymorphic microsatellite (MS) loci.The PCR products were electrophoresed on 8％ denatured polyacrylamide gel and stained using silver staining.Finally,the data were analysed and compared with the clinicopathological parameters using statistical analysis.Results In 80 IDC NOS,LOH was identified in 45 cases (56.3 ％) at least in one MS locus.MS locus D1S1310 showed the highest rate of LOH [35.7％ (25/70)].Conclusion Chromosome 1p36 might be the highly deleted region.The results of this study indicate that the chromosomal regions 1p36.23-33 might contain tumor suppressor genes associated with human breast carcinomas.

Effect evaluation of different ways of taking magnesium sulfate on bowel preparation prior to performing intravenous pyelography examination / 中国实用护理杂志

Objective To analyze optimal amount of drinking water,ways of drinking water,timelimit of drinking water on the effect of bowel preparation for intravenous pyelography (IVP) examination after oral administration of 50% magnesium sulfate 60 ml. Methods 200 patients who gave their consentfrom June 2009 to February 2010 drank 50% magnesium sulfate 60 ml for IVP check were divided into four groups. Group A: a large amount of graded oral administration of warm water 1200 ml within 1h; Group B: a small amount of oral administration of warm water 1200 ml within 1h;Group C: a large amount of graded oral administration of warm water 2000 ml within 1h; Group D: a medium amount of graded oral administration of warm water 1200 ml within 2 h. And then the bowel preparation effect of the four gro ups were analyzed. Results Through the comparison, group A was much better than the other groups on the aspects of cathartic satisfaction, the effective degree of reading films and flatulence. Conclusions To take a large amount of graded oral administration of warm water 1200 ml within 1h can improve cathartic satisfaction,the effective degree of reading films and flatulence.