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West China Journal of Stomatology ; (6): 456-459, 2004.
Artigo em Chinês | WPRIM | ID: wpr-330022

RESUMO

<p><b>OBJECTIVE</b>To evaluate the cytocompatibility of nanophase hydroxyapatite ceramics in vitro.</p><p><b>METHODS</b>Hydroxyapatite (HA) was prepared via wet method. The grain size of the hydroxyapatite in the study was determined by scanning electron microscope and atomic force microscope with image analysis software. Primary osteoblast culture was established from rat calvaria. Cell adherence and proliferation on nanophase hydroxyapatite ceramics and conventional hydroxyapatite ceramics were examined at 1, 3, 5, 7 days. Morphology of the cells was observed by microscope.</p><p><b>RESULTS</b>The average grain size of the nanophase and conventional HA was 55 nm and 780 nm, respectively. Throughout 7 days period, osteoblast proliferation on the HA was similar to that on tissue culture borosilicate glass controls, osteoblasts could attach, spread and proliferate on HA. However, compared to conventional ceramics, osteoblast proliferation on nanophase HA was significantly better after 1, 3, 5 and 7 days.</p><p><b>CONCLUSION</b>Cytocompatibility of nanophase HA was significantly better than conventional ceramics.</p>


Assuntos
Animais , Ratos , Materiais Biocompatíveis , Adesão Celular , Proliferação de Células , Células Cultivadas , Cerâmica , Durapatita , Nanoestruturas , Osteoblastos , Biologia Celular
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