Clinical effect of multi-oil fat emulsion for parenteral nutrition support in extremely low birth weight infants / 中国当代儿科杂志

OBJECTIVE@#To study the clinical effect of multi-oil fat emulsion for parenteral nutrition support in extremely low birth weight (ELBW) infants.@*METHODS@#A retrospective analysis was performed for 49 ELBW infants who were admitted from January 1, 2018 to July 30, 2020, with an age of ≤14 days on admission and a duration of parenteral nutrition of > 14 days. According to the type of lipid emulsion received, the ELBW infants were divided into two groups: soybean oil, medium-chain triglycerides, olive oil, and fish oil (SMOF) (@*RESULTS@#The 49 ELBW infants had a mean birth weight of (892±83) g and a mean gestational age of (28.2±2.3) weeks. There was no significant difference between the two groups in the incidence rates of hemodynamically significant patent ductus arteriosus, intraventricular hemorrhage, neonatal necrotizing enterocolitis, retinopathy of prematurity, bronchopulmonary dysplasia (BPD), grade Ⅲ BPD, sepsis, and pneumonia (@*CONCLUSIONS@#The application of multi-oil fat emulsion in ELBW infants does not reduce the incidence rate of complications, but compared with MCT/LCT emulsion, SMOF can reduce the severity of PNAC in ELBW infants.

Pharmacist interventions in parenteral nutrition in neonatal intensive care unit / 中国药学杂志

OBJECTIVE: To summarize the number, types, and acceptance rate of pharmacist interventions in parenteral nutrition (PN) in neonatal intensive care unit (NICU). METHODS: The pharmacist intervention in parenteral nutrition in NICU from May 1, 2014 to April 30, 2015 were summarized. RESULTS: There were 156 interventions in parenteral nutrition and the overall acceptance rate was 90.1%. The most frequent intervention was adjusting regimen. The top three types of accepted intervention were usage (100%), changing dose (95.2%), and adding medication (92.3%). CONCLUSION: Interventions in parenteral nutrition demonstrate the unique perspectiveand importance of pharmacists in NICU.

Experience of clinical pharmacists in neonatal intensive care unit / 中国药学杂志

OBJECTIVE: To introduce the experience of clinical pharmacists in neonatal intensive care unit (NICU). METHODS: The work of clinical pharmacists in NICU, including formulary decision, dosing adjustment and some special pharmaceutical care were sammarized. How to update knowledge, communicate with physicians or nurses and do research on the drug were introduced. RESULTS: Clinical pharmacists provide appropriate pharmacutical care for neonates. CONCLUSION: The pharmaceutical care plays a proper role in rational drug use in NICU.

Investigation of RHD 1227A allele in five pedigrees in Zhejiang Han population / 中华医学遗传学杂志

<p><b>OBJECTIVE</b>To analysis the genetic mode of Rh DEL phenotype and RHD 1227A allele in Zhejiang Han population through family investigations.</p><p><b>METHODS</b>Rh DEL phenotypes were identified by a serologic adsorption-elution method. Two polymerase chain reaction-sequence specific prime (PCR-SSP) methods which detectED RHD 1227A allele and Rhesus hybrid box, respectively, and a nucleotide sequencing method focused on the exon 9 of RHD 1227A allele were employed to determine the zygosity of RHD allele.</p><p><b>RESULTS</b>All five probands with Rh DEL phenotype harbored a RHD 1227A allele and had a RHD allele deletion, and they were RHD 1227A/RHd heterozygote. One of the parent members was found to contain a RHD 1227A allele and a normal RHD allele in pedigree 1, 2 and 3, respectively. Thus, they were RHD 1227A/RHD heterozygotes and presented normal D positive phenotype. The son of proband No 1. inherited the RHD 1227A allele and presented a normal D positive phenotype due to a RHD 1227A/RHD heterozygote; The offsprings of proband No. 2, No. 4, and No. 5 did not inherit RHD 1227A allele and presented a normal D positive phenotype.</p><p><b>CONCLUSION</b>RHD 1227A allele is an important genetic marker of Rh DEL phenotype; RHD 1227A is recessive to normal RHD allele and dominant to RHd allele; RHD 1227A allele is an ancestral, but not a spontaneously mutated allele.</p>

Molecular basis of Rh DEL phenotype in Zhejiang Han population / 中国实验血液学杂志

This study was purposed to investigate the molecular basis of Rh DEL phenotype. Rh DEL phenotypes were identified by a serologic adsorption-elution method, the nucleotide sequences of ten RHD exons and exon-intron boundary regions were evaluated by a RHD gene-specific PCR-SSP (PCR-SSP, polymerase chain reaction-sequence specific primer) and sequencing. The results showed that out of 122 random Rh negative donors 35 Rh DEL phenotypes were identified through serologic method, including 6 RhCCdee (17.14%), 28 RhCcdee (80.00%), and 1RhCcdEe (2.86%). Sequence analysis indicated that all DEL phenotypes harbored a RHD 1227 G > A mutation in exon 9. D zygosity test revealed that 29 DEL phenotypes (28 RhCcdee and 1 RhCcdEe) had one RHD gene deleted, and 6 DEL phenotypes (6 RhCCdee) had homogenous RHD gene. It is concluded that RHD 1227A is an important genetic marker for Rh DEL phenotype in Zhejiang Han population.