Studies on pharmacokinetics of diosmin in rats / 中国中药杂志

<p><b>OBJECTIVE</b>To establish a HPLC method for the determination of diosmin in Rats plasma and to study the pharmacokinetics of diosmin in Rats.</p><p><b>METHOD</b>Rats were given diosmin with 3 doses as 225, 325, 425 mg x kg(-1). Blood samples were collected at different times after oral administration. The plasma concentration of diosmin was determined by HPLC, and the pharmacokinetics parameters were calculated by 3p97 program.</p><p><b>RESULT</b>The typical equation of diosmin in rats plasma was Y = 3.05 x 10(-3) C + 1.55 x 10(-3), the calibration curves of diosmin was linear in the range from 0.5-100 microg x mL(-1) (R =0. 996 4). The lowest concentration of diosmin in plasma was 0. 2 g x mL(-1). Its recoveries was more than 85%, and the interday and intraday precision, which was expressed as RSD, were all less than 15%. After 3 doses oral administration of diosmin in rats, the mean plasma concentration-time curves were found to fit one compartment model, and the main pharmacokinetics parameters were obtained.</p><p><b>CONCLUSION</b>It is first time to establish the HPLC method to determine the concentration of diosmin in rats plasma, and the method described in this report has high sensitivity and selectivity, and it was suitable for pharmacokinetics studies of diosmin. The internal process of diosmin in rats is fit to one compartment model.</p>

Fingerprint of Sanhuang Xiexin Decoction by HPLC / 中草药

Objective To establish the HPLC fingerprint of Sanhuang Xiexin Decoction and provide a method to study the potential basis and the changing of the chemical component for it in different compati- bility.Methods An HPLC method was established with Shimadzu ODS column(150 mm?4.6 mm, 5?m),the mobile phase was methanol-water-0.1% phosphoric acid(0.01 mol/L potassium phosphate monobasic,pH 2.8)as gradient elution,the flow rate was 1.0 mL/min,and the detection wavelength was 260 nm.Through comparing and analyzing the relative retention time of this decoction and of its composi- tion which are positive and negative control fingerprints,the main chromatographic peak origins were con- firmed;The correlated chromatographic peaks were identified by contrasting chromatographic peak reten- tion time and adding reference substances to the sample.Results All tested samples contained the 32 common peaks,the relativity of them were analyzed and 11 peaks were indicated.The similarity of ten batches of samples exceeded 0.92.Conclusion This method shows sensitive and good repeatability,all of the contents are separated well.It is used to determine Sanhuang Xiexin Decoction and its relative prepara- tions.