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ObjectiveTo evaluate the inhibiting effects of mitomycin C（MMC） on fibroblasts of human pterygium （HPF） in vitro．MethodsThe fibroblasts derived from the 5 th to 8 th generation of the cultured tissues in human pterygium．These fibroblasts were exposed to culture plates with MMC at 6 gradient concentrations，adjusting the concentration value of the culture medium to 105，104，103，102，10 and 1 μg/L．The cells had been treated with PBS in the control group．After 48 h of incubation，the absorbency had been determined individually by MTT［3-（4，5-dimethylthiazol-2-yl）-2，5-diphenyl-tetrazolium bromide］ method.The inhibitory rates were calculated accordingly．All the data were analyzed with student t test and linear regression．ResultsThe extensive breakage of fibroblasts was observed．There was a statistical difference between the tested group and control group in absorbency (P＜0.05)，especially at the concentrations of more than 100 μg/L (P＜0.01)．The degree of growth inhibition increased with exposure of cells to MMC over 48 h．It showed that there is a positive correlation between the doses of MMC and the antiproliferative effects on HPF．ConclusionMMC significantly inhibites the proliferation of HPF in a dose-dependent manner．
RESUMO
AIM: To observe the effect of diterpenoid compound 5F of Pteris semipinnate L on apoptosis in cultured human pterygium body fibroblasts(HPFs).METHODS: Fibroblasts collected from human pterygium body were cultured in vitro with 5F at different time point.Transmission electron microscope(TEM) was used to observe the ultrastructure changes of the HPFs.The changes of the cell cycle and apoptosis of HPFs were evaluated with flow cytometry(FCM).RESULTS: 5F at the concentration of 32 mg/L induced HPF apoptosis at various time points.CONCLUSION: 5F induces HPFs apoptosis.Apoptotic cells are evolved to necrosis with the prolongation of the time.These findings indicate that 5F has a potential clinical value.