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BACKGROUND:The aim of present study is to demonstrate that volatile oil of flos magnoliae biondii pamp(VOMbp) possess anti-immunology inflammatory property.OBJECTIVE:To explore the effect of VOMbp on adjuvant arthritis in rats.DESIGN:Grade sampling,randomized controlled quantitative analysis.SETTING:Teaching and Researching Department of Pharmacology,Chengdu University of Traditional Chinese Medicine.MATERIALS:This study was carried out at the Teaching and Researching Department of Pharmacology,Chengdu University of Traditional Chinese Medicine from December to April in 1999. Totally 120 Wistar rats were involved.METHODS :Wistar rats were randomly divided into 4 groups :model control group, 0.28 mL/kg and 0.14 mL/kg VOMbp group,and 25 mg/kg dex-amethasone group. Allergy were induced in all rats through intra-cutaneous injection of Freund's adjuvant (0.1 mL/rat)at metatarsus so as to establish multiple arthritis model in rat. According to different dosage,the experiments were carried out in three batches with 10 rats in each group.Rats in the first batch were given VOMbp three days before inducing allergy for consecutwe 3 days,in order to observe the influence of VOMbp on adjuvant induced acute non-specific inflammatory;Rats in the second batch were given VOMbp on the 8th day sensitization allergy for consecutive 7days so as to explore the protective effect on secondary pathological changes;rats in the third batch were administratedon the 19th day after sensitization for consecutive 7 days for study in therapeutic effect on sec ondary pathological changes. Meanwhile foot tumefaction and the level of prostaglandin E2in ankle arthritis were observed.MAIN OUTCOME MEASURES: ① Effect of VOMbp on the adjuvant induced acute non-specific inflammatory. ② Preventive effect on adjuvant arthritis. ③ Therapeutic effect on adjuvant arthritis.RESULTS :① VOMbp could significantly suppress the primary foot tume faction. ②Moreover VOMbp of preventive dosage could most significantly suppress the secondary arthritis tumefaction [(0.231±0.065)mL of 0.28 mL/kg group and ( 0.206±0.082 ) mL of 0.14 mL/kg group] ,accompanied by PG-E2 decrement in tumefaction tissues [(0.133 2±0.057 2), (0.138 5 ±0.042 6)OD278nm correspondingly].③ No obvious effect of VOMbp of therapeutic dosage on secondary inflammatory tumefatcion of arthritis could be observed in 0.14 mL/kg group,but obvious decrement of prostaglandin E2 could be observed in edema tissue;While 0.28 mL/kg VOMbp exerted most significant or significant influence on above parameters. CONCLUSION:VOMbp possess inhibiting effect on Freund's adjuvant induced primary foot tumefaction and secondary arthritis,with the preventive effect better than therapeutic effects.The therapeutic effect was proved better in higher dosage group.
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objective To study the anti-inflammation effect of volatile oil from ramulus cinnamomi(VORC).Methods Anti-inflammation effect was studied with the methods of mice auricular swelling,mice celiac capillary permeability,rat hind paw edema and acute pneumonia model.Results VORC had an inhibitory effect on acute inflammation of mice induced by xylene,celiac capillary permeability of mice induced by acetic acid,edema of rat hind paw induced by carrageenan,acute pulmonary inflammation of rat induced by LPS.Conclusion VORC has a markedly anti-inflammation action.
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The methods and technology of immunopharmacology for Chinese Materia Medica have been progressing rapidly in recent years. The regulation of Chinese Materia Medica on the activity of cytokine and effector cells is reviewed in this article. Many clinical studies indicate that Chinese Materia Medica can regulate the production and activity of IFN, ILs, TNF, etc., and affect the cytotoxieity of NK. LAK, CTL, etc. The immunopharmcological research of Chinese Materia Medica in molecular and cellular levels is useful for explaining the mechanism of whole regulation and two-way regulation depending on the function of the body. In this article, the relations between quantity and effect, the study in vivo and in vitro, and the whole drug and its component are also reviewed.
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AIM To study the mechanism of puerarin against arrhythmias. METHODS Standard microelectrode intracellular recording technique was used RESULTS ①Puerarin 0 005, 0 01,0 015 mmol?L -1 prolong the action potential duration at 50% and 90% of repolarization (APD 50 ,APD 90 ) respectively APD 50 was prolonged from (176 43?51 37) ms to (192 86?60 82) ms ( n=7, P