RESUMO
ObjectiveTo evaluate the effectiveness of Lutongning granules in the treatment of trigeminal neuralgia in animal models and study its mechanism of action， so as to provide laboratory data support for the clinical application of Lutongning granules and precise treatment. MethodMale SD rats were randomly divided into normal group， model group， carbamazepine group （0.06 g·kg-1·d-1）， high-dose Lutongning group （2.70 g·kg-1·d-1）， and low-dose Lutongning group （1.35 g·kg-1·d-1） according to the stratified basic mechanical pain thresholds， with 10 rats in each group. A trigeminal neuralgia model of rats was prepared by injecting 30% talc suspension into the infraorbital foramen area of the rat. The drug groups were administered 10 mL·kg-1 of drugs by gavage after 2 h of modeling. The normal group and the model group were administered distilled water by gavage under the same conditions once a day for 10 consecutive days. Von Frey brushes were used to determine the mechanical pain threshold of rats. A fully automated blood and body fluid analyzer was employed to detect the blood routine of rats. Hematoxylin and eosin （HE） staining was utilized to detect the pathological changes in the trigeminal ganglion and medulla oblongata tissue. Transmission electron microscopy was used to scan the ultrastructure of the medulla oblongata tissue. Enzyme-linked immunosorbent assay （ELISA） was used to detect the levels of inflammatory factors interleukin （IL）-1， IL-6， IL-8， tumor necrosis factor （TNF）-α， neuropeptide substance P， and β-endorphins （β-EP） in the serum of rats， and Western blot was used to detect the protein expression levels of IL-1β， purinergic receptor P2X7 （P2X7R）， and phosphorylated p38 mitogen-activated protein kinase （p-p38 MAPK）. ResultCompared with that in the normal group， the pain threshold of rats in the model group was significantly lower （P<0.01）. The absolute value of neutrophils （NEUT#） and the percentage of neutrophils （NEUT） were significantly improved， and the percentage of lymphocytes （LYMPH） was significantly reduced （P<0.01）. The serum levels of IL-1， IL-6， IL-8， and TNF-α were significantly increased （P<0.01）. SP content in brain tissue was significantly increased， and β-EP content was significantly decreased （P<0.01）. The relative protein expression of IL-1β， P2X7R， and p-p38 MAPK was significantly increased （P<0.05）. HE staining and transmission electron microscopy results of medulla oblongata tissue revealed neuronal degeneration， mild proliferation of microglial cells， reduction in the number of myelinated nerves， and obvious demyelination. The trigeminal nerve fibers of rats were disarranged， and some nerve fibers showed vacuolization. Axons were swollen， and Schwann cells proliferated. Demyelination was observed. Compared with the model group， each administration group significantly increased the pain threshold of rats （P<0.05， P<0.01）， reduced NEUT# and NEUT， and elevated LYMPH （P<0.05， P<0.01）. The administration group significantly decreased the levels of IL-1， IL-6， IL-8， and TNF-α in serum and SP in brain tissue （P<0.01） and increased the level of β-EP （P<0.01）. They significantly down-regulated the protein expression of IL-1β， P2X7R， and p-p38 MAPK（P<0.05， P<0.01） and significantly ameliorated the pathological changes in medulla oblongata tissue and trigeminal nerves of rats. ConclusionLutongning Granules had significant therapeutic effects on trigeminal neuralgia induced by injection of talc into the infraorbital foramen of model rats， and the mechanism may be related to amelioration of P2X7R-mediated neuroinflammation and inhibition of demyelination of myelinated nerves.
RESUMO
Objective:In order to allow the large instruments to play the greatest role in scientific research management and technology coverage.Methods:Texts review of the opening and sharing of large-scale instrument platforms of domestic and foreign universities and scientific research institutions was conducted, combined with the experience of more than 10-year operation of the technology platform of State Key Laboratory of Natural and Biomimetic Drugs of Peking University.Results:There are particular problems in the opening and sharing of large-scale instrument technology service platforms at this stage. Through building the hierarchical management of large instruments and equipment, building an efficient and flexible shared management information system, introducing high-level instrument management and high-level talents, and strengthening the security management of the shared platform, State Key Laboratory of Natural and Biomimetic Drugs of Peking University realized the opening and sharing of large-scale instrument technology service platforms. We consider that the current large-scale instrument technology service platforms of universities and research institutes are necessary and feasible to be shared. It is greatly significant to improve the utilization rate of scientific research instruments and to innovate to the ability of the whole society.Conclusions:At this stage, efficient management, system capabilities, and team building can effectively promote the opening and sharing of large-scale instrument technology service platforms.
RESUMO
Objective:In order to study the necessity of introducing PhD students into the large-scale instrument platform and how to make them play a better role in work through management.Methods:The status of technicians in large-scale instrument and equipment platforms of domestic and foreign universities and scientific research institutions were investigated; combined with the experience of more than 10 years of operation of the technology platform of State Key Laboratory of Natural and Biomimetic Drugs of Peking University, the analysis was carried out.Results:In recent years, the large-scale instrument platforms of major universities and scientific research institutions have strengthened the introduction of high-level talents and the construction of the team. Maintain the stability of the instrument service staff through management measures such as rank promotion and salary enhancement.Conclusions:High-precision instruments are the foundation of the instrument technology platform, and well-educated talents are the core of the instrument technology platform. Only when more PhD students join the technology platform, can the high-precision instruments and equipment exert their maximum functions and promote scientific research.