Clinical study of telbivudine on PCR technique to detect the DNA of Mycobacterium tuberculosis / 中国基层医药

ObjectiveTo value fluorescent quantified PCR (polymerase chain reaction) technique to detect the DNA of Mycobacterium tuberculosis from clinical patients.MethodsDetect the DNA of TB from the sputum of 80 active tuberculosis and hydrothorax of 40 tuberculous pleuritis.At equal pace,make a comparison to proceed microscopic examination and cultivation.ResultsWith fluorescent quantified PCR,56.2％ (45/80),27.5％ (11/40)positive detection from active tuberculosis and tuberculous pleuritis respectively.16.2％ ( 13/80 ),0.0％ positive specimen were detected with microscopic examination in two group of patients.Cultivation got 37.5％ (30/80),2.5％(1/40) positive detection respectively.ConclusionFluorescent quantified PCR was more sensitive and specific than traditional methods,and valuable in the microscopic examination and cultivation negative patients especially.

Study on the relationship between children obesity and adiponectin,leptin,high-sensitive C-reactive protein / 中国医师杂志

Objective To explore the relationship between children obesity and adiponectin(ADPN),leptin(LP),and high-sensitive C-reactive protein(hsCRP).Methods Seventy-one obese volunteers as obesity group and 30 normal volunteers who matched obese volunteers in age and sex as control group were selected from 13 702 children and adolescents of 2 to 18 years old in Xiangtan city by sampling survey.The body mass index(BMI),ADPN,LP,hsCRP,fasting blood-glucose(FBG),and fasting pure insulin(INS) were checked respectively.The homeostasis model assessment for insulin resistance(HOMA-IR) and the quantitative insulin-sensitivity check index(QUICKI) were calculated.The difference between the two groups was compared and the relationship of all indexes was analyzed.Results The values of ADPN and QUICKI in obesity group were significantly lower than those of control group(P