RESUMO
Objective To explore the association between ACTN3 gene R577X polymorphism and serum lipid levels in an elderly Chinese Han population.Methods This study was based on the ageing arm of The Rugao Longevity and Ageing Study (RtLAS).Genotyping was performed by Taqman MGB method.Lipids includedtotal cholesterol (TC),triglyceride (TG),high-density lipoprotein cholesterol (HDL-C) and low-density lipoprotein cholesterol (LDL-C).Cut points of dyslipidemia were based on that reported in the NCEP ATPIII.We explored the associations between R577X polymorphism loci and dyslipidemia by using analysis of covariance and logistic regression analysis.Results Overall 1 618 samples was included (741 males and 877 females) in this study.Covariance analysis found that TC level of RR,XX and RX genotype in the general population were 1.34,1.37,1.43 mmol/L with an increasing trend (P =0.024);TC and LDL-C levels of RR,XX and RX genotype (TC:5.13,5.29,5.43 mmol/L,P =0.004;LDL-C:2.76,2.88,3.00 mmol/L,P =0.004) were significantly different in females.Logistic regression analysis showed that the increased copies of X allele were significantly associated with increased TC and LDL-C levels in the general population and females.For the general population,TC:OR=1.184,95％CI:1.030-1.361,P =0.018;LDL-C:OR =1.334,95％CI:1.101-1.588,P=0.003;For females,TC:OR=1.332,95％ CI:1.102-1.616,P =0.003;LDL-C:OR =1.549,95 ％CI:1.208-1.986,P =0.001.After adjusting for other covariates,the above associations remained significant.Conclusions ACTN3 gcnc R577X polymorphism is associated with plasma TC and LDL-C levels in elderly woman of Han populations in Rugao,China.
RESUMO
[Objective]To observe the influence of Yigu oral liquid on inhibiting the pain in ovariectomized rats by detecing the pain threshold, bone densi-ty, serum inflammatory factor, vascular endothelial function(ET) and platelet activating capacity(CD62p and CD63).[Method]80 SD rats were randomly di-vided into four groups of sham group, Yigu oral liquid group, Miacalcic group,control group. There were 20 in each group.Yigu oral liquid group,Mia-calcic group,control group were ovariectomized,sham group underwent sham operation. After confirming the successful model of osteoporosis, sham group was administrated with saline, Miacalcic saline group was administrated with Miacalcic by intramuscular injection ,Yigu oral liquid group was administrated with Yigu oral liquid ,saline and control group was administrated with saline.The indicators of pain threshold,bone density,vascular endothelial function (ET) and platelet activating capacity(CD62p and CD63), serum inflammatory factors as COX-2, PGE2, cAMP were respectively detected. [Results] The levels of peripheral inflammatory factors as COX-2, PGE2, cAMP , vascular endothelial function(ET) and platelet activating capacity(CD62p and CD63)of Yigu oral liquid group decreased significantly compared with the control group( P<0.05). The level of pain threshold and bone density of Yigu oral liquid group improved significantly compared with the control group(P<0.05).[Conclusion]Yigu oral liquid can inhibit osteoporosis pain by improving the level of pain threshold ,bone density,and decreasing the levels of COX-2, PGE2, cAMP, vascular endothelial function(ET) and platelet activating capacity (CD62p and CD63).