RESUMO
Objective To analyze the effect of duloxetine on S100B and signal pathway ERK1/2-NF-κB expression in hippocampus in depression rat.Methods Chronic unpredictable mild stimulation was used to establish depressive model rats (n=50).They were randomly divided into no-intervention group (n=10),different treatment time of duloxetine group (C,D,E,F group,10 rats in each group)and then 10 normal rats were selected as control group.Behavior tests including open-field test and the saccharine preference test were used to test the behavioral change of rats after 28 days intragastric administration.Western blot was used to detect S100B,t-ERK1/2,pERK 1/2,t-NF-κB and pNF-κB expression in hippocampus.Results In open-field test,the crossing score,rearing score and latency of the rats in E,F group were (69.68± 14.61) and (70.66± 11.53) score,(20.94 ± 10.92) and (20.32±8.85) score,(1.1±0.4)s and(1.0±0.4) s respectively,and showed no significant difference with those of control group ((71.19±12.08) score,(20.42±8.76) score,(1.0±0.3)s) after 28 d intragastric administration (P＞0.05),while the level score,vertical score were significantly higher than those in depressive model (P＜ 0.05).In the saccharine preference test,the rats in E,F and control group exhibited increased saccharin preference compared with depressive model rats (P＜0.05).The rats in E,F and control group exhibited increased S100B,pERK1/2 and pNF-κB expression in hippocampus compared with depressive model rats (P＜0.05).Conclusion Duloxetine improves the behavioral ability of depression rat and exerts effect after 2 weeks.The ERK1/2-NF-κB signal pathway in hippocampus may participate in this mechanism.