Preparation and In Vitro Characterization of Gelatin Methacrylate for Corneal Tissue Engineering

BACKGROUND@#Corneal disease is second only to cataract considered as the leading cause of blindness in the world, with high morbidity. Construction of corneal substitutes In Vitro by tissue engineering technology to achieve corneal regeneration has become a research hotspot in recent years. We conducted in-depth research on the biocompatibility, physicochemical and mechanical properties of rat bone marrow mesenchymal stem cells (rBM-MSCs)-seeded gelatin methacrylate (GelMA) as a bioengineered cornea. @*METHODS@#Four kinds of GelMA with different concentrations (7, 10, 15 and 30%) were prepared, and their physicchemical, optical properties, and biocompatibility with rBM-MSCs were characterized. MTT, live/dead staining, cell morphology, immunofluorescence staining and gene expression of keratocyte markers were performed. @*RESULTS@#7%GelMA hydrogel had higher equilibrium water content and porosity, better optical properties and hydrophilicity. In addition, it is more beneficial to the growth and proliferation of rBM-MSCs. However, the 30%GelMA hydrogel had the best mechanical properties, and could be more conducive to promote the differentiation of rBM-MSCs into keratocyte-like cells. @*CONCLUSION@#As a natural biological scaffold, GelMA hydrogel has good biocompatibility. And it has the ability to promote the differentiation of rBM-MSCs into keratocyte-like cells, which laid a theoretical and experimental foundation for further tissue-engineered corneal stromal transplantation, and provided a new idea for the source of seeded cells in corneal tissue engineering.

Research advances in the effects of magnesium ion on the development of preterm infants / 中华实用儿科临床杂志

Magnesium ion (Mg2+ ) plays an important role in maintaining the life and health of the body.In preterm infants, the protective effect of prenatal Mg2+ supplementation on the nervous system of preterm infants has been widely recognized.Researchers begin to pay attention to the effects of serum Mg2+ level on the development of nervous system and effects of prenatal exposure to Mg2+ on the non-nervous system in preterm infants.The effects of Mg2+ on the development of neural and non-neural system in preterm infants were summarized.

Research advances in the effects of magnesium ion on the development of preterm infants / 中华实用儿科临床杂志

Magnesium ion(Mg2+)plays an important role in maintaining the life and health of the body. In preterm infants,the protective effect of prenatal Mg2+ supplementation on the nervous system of preterm infants has been widely recognized. Researchers begin to pay attention to the effects of serum Mg2+ level on the development of nervous system and effects of prenatal exposure to Mg2+ on the non-nervous system in preterm infants. The effects of Mg2+ on the development of neural and non-neural system in preterm infants were summarized.

Changes and clinical significance of platelet activation and four platelet parameters in diabetic retinopathy / 眼科新进展

Objective To explore the relationship between platelet activating factors and four platelet parameters in diabetic retinopathy (DR).Methods Eighty patients in our hospital were chosen from January 2015 to December 2015,regular ophthalmological examination and fundus fluorescence were performed on all cases.On the basis of international clinical diabetic retinopathy classification standard,the patients were divided into four groups:No DR group with 20 cases,non-proliferative diabetic retinopathy (NPDR) group with 20 cases,and proliferative diabetic retinopathy (PDR) group with 20 cases.20 healthy people who received physical examination during the same period were recruited as the control group.The flow cytometry instrument was used to determinate platelet activating factor CD62p and CD63,automatic blood analyzer was used to determinate platelet parameter which including blood platelet count (PLT),mean platelet volume (MPV),plateletcrit (PCT) and platelet distribution width (PDW),and the changes of platelet activation factor,parameters and its clinical significance in DR were discussed.Results With the development of DR,CD62p,CD63,MVP were numerical gradually increased,and PLT were numerical gradually reduced,the differences were statistically significant (all P ＜ 0.05);Compared with control group,CD62p,CD63,MVP were increased and PLT were reduced in other group,the differences were statistically significant (all P ＜ 0.05);Compared with pure diabetes group,CD62p,CD63,MVP were increased and PLT were reduced in NPDR group and PDR group,the differences were statistically significant (all P ＜ 0.05);Compared with NPDR group,CD62p,CD63,MVP were increased and PLT were reduced in PDR group,the differences were statistically significant(all P ＜ 0.05).PDW were numerical gradually increased in control group,pure diabetes group,NPDR group,PDR group,the differences were statistically significant(all P ＜ 0.05);Control group compared with pure diabetes group,NPDR group compared with PDR group,the differences in PDW was not statistical significant (all P ＞ 0.05);But compared with control group or pure diabetes group,PDW of NPDR group and PDR group increased,the differences were statistically significant(all P ＜ 0.05).The differences in PCT among groups was not statistically significant(all P ＞ 0.05).Conclusion The platelet activating factor CD62p,CD63 and parameters of platelet show differences in each diabetic retinopathy period,platelet activating factor in the occurrence and development of diabetic retinopathy have important significance.Monitor the platelet activating factor and parameters of platelet can help surveillance of the disease.

Investigation of oral hygiene of the adults in Guizhou province / 实用口腔医学杂志

Objective:To investigate the status of oral hygiene behavior of the people of 1 8 yeas old and above in Guizhou prov-ince.Methods:A total of 9 280 adults were included by multi-staged sampling from 1 2 counties in Guizhou province of China.The double entry data were processed by Epiinfo 3.5.1 and statistically analysed by SPSS 1 9.0.Results:The survey of 9 280 subjects were permanent residents from 201 0 -201 1 .1 5.9% of the total subjects experienced dental visit,those from city and contrside were 23.0%,1 2.5%;male and female were 1 4.4%,1 7.1 %,respectively.5.6% of the them experienced tooth scaling,those from city and countryside were 9.9% and 3.4%,male and female were 4.9%,6.2% respectively.The subjects with 1 time and 2 time tooth brushing a day were 58.1 % and 28.6%,respectively.6% of the subjects did not brush teeth,mainly were those over 60 years (21 .3%),those of city residents was significantly lower than countryside(7.0% vs 4.0%).Conclusion:The oral heal behavior of Guizhou residents is poor,the education of oral health should be strengthened.

Reevaluation on the mechanical properties of dentine microstructure / 医用生物力学

Objective:It is demostrated that the porous protein-mineral mechanics model could provide more accurate prediction for biomaterial properties of dentine compared with the other established models.This paper would use the model to reevaluate the mechanical properties and its interacting mechanism of human dentine.Method:By using a porous proteinmineral mechanics model,the effect from the interactions between tubules,pedtubular and intertubular matrix on dentine microstructure was discussed.Results:The dentinal micromechanical properties were dependent on the tubular direction,and the absolute values of the stresses derived from the hydraulic and gas tubular pressures increased parabolically with theincreasing diameter of the tubules.It was also found that the effective elastic constants of the dentine microstructure would vary with the aging and the distribution of mineral and collagen within peritubular and intertubular matrix of detine.Conclusions:The theoretical analyses provided in this paper demonstrated that the microstructural characteristics of tubules,peritubular and intertubular dentinal matdx could have different influences on the micromechanical properties of human dentine,which showed the validity of porous protein-mineral mechanics model,and the limitation of some models that neglected the interacting mechanism.

Sonograms of Tuberculous Peritonitis / 中国医科大学学报

Objective To study the sonograms of tuberculous peritonitis and improve the diagnostic accuracy of this disease.Methods We retrispectively analyzed 138 cases of tuberculous peritonitis diagnosed by ultrasonically guided biopsy,postoperative pathology,and clinical manifestation.The characteristics of the sonograms were analyzed,including the amount and character of ascites,the changes in greater omentum,peritoneum,and mesenterium.the enlarged celiac lymph nodes,and the organ involvement.Results The sonograms of tuberculous peritonitis were characterized by thickening of greater omentum with different degrees of ascites,which usually presented cerebral fissure sign under high-frequency ultrasound,together with aggregation of intestines,thickening of peritoneum,enlarged celiac lymph nodes,hepatomegaly,splenomegaly,and pleural effusion.Conclusion Ultrasonography is of great clinical significance in the diagnosis of tuberculous peritonitis and the guidance of greater omentum biopsy.

Diagnostic value of ultrasonic scoring of benign and malignant breast nodules / 中国介入影像与治疗学

Objective To assess the diagnostic value in breast nodules with ultrasontic scoring.Methods Benign and malignant breast nodules were studied with ultrasonic scoring and compared with pathologic results.Results The 2D-ultra-sontic scoring and total ultrasontic scores of benign breast nodular lesions had significant difference compared to malignant breast nodular lesions (P<0.01).Ultrasontic scores of malignant breast nodular lesions were higher than those of benign breast nodular lesions (P<0.01).According to the ROC curve and Youden index,2D-ultrasonic scores ≥11 could be considered as the critical point for the diagnosis of malignant breast nodular lesions,the total ultrasonic scores ≥12 could be considered as the critical point for diagnosis of malignant breast nodular lesions.Conclusion Ultrasonic scoring for breast nodular lesions may be useful for improving the diagnosis accuracy of the benign and malignant breast nodular lesions.

Categorization and anorectal manometry in chronic constipation / 中华消化杂志

Objective We categorized chronic constipation according to colonic transit time (CTT) and transit index (TI), and investigated the abnormalities of anorectal motility and sensory function in patients in different categories. Methods Each subject ingested 20 radiopaque markers, and abdominal X rays were obtained at 72 h. Chronic constipation was categorized according to CTT and TI. Anorectal manometry was carried out in all subjects to investigate the abnormalities of anorectal motility and sensory function in patients in different categories. Results Chronic constipation was divided into four types: normal transit constipation (NTC), slow transit constipation (STC), outlet obstructive constipation (OOC), and mixed type constipation (MC). Patients with constipation displayed significantly lower anorectal resting pressure, squeezing pressure and larger rectal maximum tolerate volume than controls ( P