Identification of chemical constituents in qiliqiangxin capsule by UPLC-Q-TOF/MS(E) / 药学学报

In order to clarify the chemical constituents in Qiliqiangxin capsule, a rapid ultra-performance liquid chromatography/orthogonal acceleration time-of-flight mass spectrometry (UPLC-Q-TOF/MS(E)) method was established. Forty peaks were identified on line using this method. The herbal sources of these peaks were assigned. The results implied that triterpenoid saponins, flavonoid glycosides, C21-steroids and phenolic acids were included in the main components of Qiliqiangxin capsule. The method is simple and rapid for elucidation of the constituents of Qiliqiangxin capsule and the results are useful for the quality control of Qiliqiangxin capsule.

RECONSTRUCTION AND FUSION WITH MRI OF CAUDATE NUCLEUS, LENTIFORM NUCLEUS AND THALAMUS / 解剖学报

Objective To reconstruct caudate nucleus,lentiform nucleus and thalamus three-dimensionally,fuse these neural structures with (patient's) radiological images and provide anatomical data for diagnosis and treatment of neurosurgical diseases. Methods Chinese Virtual Human dataset was segmented using image-transparency method at first and each image was smoothed by eroding and dilating, then fused neural structures with MRI,the neural structures and brain in MRI were reconstructed and displayed using surface rendering and volume rendering lastly. Results The reconstructed neural structures were smooth,natural and realistic.Their shapes and positions was clearly displayed and could be rotated, observed in any direction.On the fused images,the neural structures and tumor could be seen at the same time.Conclusion The reconstructed structures and fused images have great reference value to teaching and learning and diagnosis and treatment of neurosurgical diseases.

Apoptosis and expression of relative genes in early pregnant chorionic villi and decidua / 中华妇产科杂志

Objective To investigate the effects of mifepristone on apoptosis and expression of relative genes in early pregnant chorionic villi and decidua Methods The specimen of early pergnant chorionic villi and decidua obtained from 10 cases of requesting temination of pregnancy by curettage, 20 cases of mifepriston contragestation The paraffin sections were used to determine apoptotic cells by TdT mediated dUTP biotin nick end labeling method, to identify bcl 2, bax, fas, fasL and proliferating cell nuclear antigen (PCNA) by immunohistochemistry, to demonstrate fas and fasL mRNA by in situ hybridization Results In normal early pregnant specimens, apoptotic cells were mainly observed in syncytiotrophoblast, but not in cytotrophoblast cells, occationally seen in decidua cells The antigen of bax, fas, fasL were present in syncytiotrophoblast cells and decidua with lower amount While bcl 2 antigen staining was strong in cytotrophoblastic cells and in decidua PCNA protein was present in cytotrophoblastic and decidual cells only In the specimens treated with mifepristone, apoptotic cells were increased in syncytiotrophoblastic cells of villi and visualized in decidua cells The expression of fas, fasL and bax was also higher than that of nomal Conclusions Mifepristone increased apoptosis in syncytiotrophoblastic and decidua cells, but had no effect on the expression of bcl 2 and PCNA