RESUMO
Objective To determine the prevalence of overweight and obesity among children and adolescents in Shenyang and the relationship between the birth weight to overweight and obesity in the childhood aged 7 to 17 years.Methods A stratified cluster representative sample of 5800 children and adolescents aged 7 to 17 years from 27 schools among 3 regions in Shenyang was selected.The study was carried out by using questionnaire about birth weight and physical examination including weight and height.Overweight and obesity were defined according to body mass index cutpoint.The body mass index classification criteria recommended by the Chinese Working Group on Obesity for Children(WGOC) was adopted,and the relationship between birth weight and overweight or obesity in children and adolescents were analyzed by collecting the information associated with overweight or obesity.Results The overall combined prevalence of overweight and obesity was 21.13％ with obesity as 7.12％ based on the WGOC criteria,and children of 10 to 12 years old were high-risk population groups.The risk of a male becoming overweight or obesity was 2.01 times higher than the risk of a female.The prevalence of overweight and obesity in Shenyang were level Ⅱ,especially for males the incidence of overweight and obesity of high birth weight babies was 1.73 (95％ CI:1.51-2.87) times higher than that of normal birth weight babies,while the incidences of overweight and obesity between low birth weight babies and normal birth weight babies were not statistically different.Conclusions The study indicated that the prevalence of overweight or obesity children and adolescents are level Ⅱ in Shenyang.There is high risk to be overweight and obesity in adolescences and especially for males.To decrease overweight and obesity in children and adolescents we should pay greater attention to fetus period.
RESUMO
To explore the apoptotic effect of simvastatin on K562 cells through endoplasmic reticulum stress, morphological change of apoptotic cells was observed by Hoechst33258 fluorescent staining under fluorescent microscope. Apoptosis rate of cells was determined with annexinV-FITC/PI double staining by flow cytometry; Intracellular calcium concentration ([Ca2+]i) was measured by laser scanning confocal microscope (LSCM); The expression levels of glucose regulated protein 78 (GRP78) and calpain gene mRNA were determined by RT-PCR; The expression levels of caspase-3, -6, -7, -9, -12, calpain and GRP78 proteins were evaluated by Western blotting. In this study, K562 cells treated with simvastatin for 72 h exhibited typical morphological change of apoptosis cells. After 72 h exposed to 10, 20, 30 micromol x L(-1) simvastatin, the apoptotic rates of K562 cells were 12.41%, 19.08% and 23.41%, respectively. Simvastatin induced the increase of [Ca2+]i in K562 cells, fluorescent intensities were 43, 54, and 64, respectively. The expression levels of GRP78 and calpain gene mRNA were up-regulated. The cleavage and activation of caspase-3, -6, -7, -9, -12 and upregulation of GRP78 expression were determined by Western blotting. These findings suggest that endoplasmic reticulum is an important pathway of apoptosis in cells and participates simvastatin-induced apoptosis in K562 cells. It is implied that simvastatin may be suitable for clinical usage in the treatment of myeloma patients.