RESUMO
Head and neck squamous cell carcinoma(HNSCC) is the sixth most common cancer among men in the developed world affecting the tongue, pharynx, larynx and oral cavity. HNSCC is thought to represent a multistep process whereby carcinogen exposure leads to genetic instability in the tissue and accumulation of specific genetic events, which result in dysregulation of proliferation, differentiation, and cell loss and the acquisition of invasive capacity. Despite therapeutic and diagnostic progress in oncology during the past decades, the prognosis of HNSCC remains poor. Thus it seems that finding a biological tumor markers which will increase the early diagnosis and treatment monitoring rates, is of paramount importance in respect to improving prognosis. In an effort to identify gene expression signatures that may serve as biomarkers, this study several genes were selected, such as H3,3A, S100A7, UCHL1, GSTP1, PAI-2, PLK, TGFbeta1 and bFGF, and used 7 HNSCC cell lines that were established various anatomical sites, and also 17 other cancer cell lines were used for control group using real-time quantitative RT-PCR and immunocytochemical analysis with a monoclonal antibody. In this study, S100A7 showed a clearly restricted occurrence in tongue originated cell line, and GSTP1 expression level in the pharynx originated cell line was very increased, relative to corresponding other cell lines. These results suggest that S100A7 and GSTP1 genes' expression can occur during tongue and pharynx originated head and neck tumorigenesis and that genetic change is an important driving force in the carcinogenesis process. This data indicate that S100A7 and GSTP1 expression pattern in HNSCC reflect both diagnostic clue and biological marker. And this is provides a foundation for the development of site-specific diagnostic strategies and treatments for HNSCC.
Assuntos
Humanos , Masculino , Biomarcadores Tumorais , Carcinogênese , Carcinoma de Células Escamosas , Linhagem Celular , Diagnóstico Precoce , Cabeça , Laringe , Boca , Pescoço , Faringe , Inibidor 2 de Ativador de Plasminogênio , Reação em Cadeia da Polimerase , Prognóstico , Língua , TranscriptomaRESUMO
The treatment of TMJ ankylosis poses a significant challenge because of technical difficulties and high incidence of recurrence. TMJ ankylosis has been treated by excision and total joint reconstruction with alloplastic, allogeneic, autogenous materials as interpositional materials. The temporalis myofascial flap had been considered to be a successful interpositional material, due to its anatomical, topographical, and functional properties. This study evaluated the efficacy of the temporalis myofascial flap for nine TMJs (five patients) through the preauricular approach and coronoidectomy. Radiographic and physiologic long term result was investigated in this study. The result reveals that the temporalis myofascial flap is a good autogenous tissue satisfying the criteria of an ideal interpositional material, which offers a material that fulfills the physiological function of the disc. In spite of favorable functional outcome, mild postoperative openbite tendency remains another challenge.
Assuntos
Anquilose , Artroplastia , Incidência , Articulações , Mordida Aberta , Recidiva , Articulação TemporomandibularRESUMO
Assuntos
Humanos , Medula Óssea , Implantes Dentários , Mandíbula , Osteoporose , Coluna Vertebral , DenteRESUMO
Assuntos
Adulto , Humanos , Angiofibroma , Biópsia , Cisplatino , Doxorrubicina , Tratamento Farmacológico , Seguimentos , Boca , Metástase Neoplásica , Osteossarcoma , Prognóstico , Recidiva , Pele , Base do Crânio , Taxa de Sobrevida , Osso TemporalRESUMO
The purpose of this study was to evaluate the administration period of the prophylactic antibiotic and anti-inflammatory drug used to reduce postoperative infection and edema, as compared with normal tissue healing period after operation. Author selected the 98 patients who had been performed maxillofacial bone fracture surgery, orthognathic surgery, sort tissue surgery, removal of miniplate and screw, and cyst operation, among 159 patients admitted to the department of oral and maxillofacial surgery of the one general hospital in Daegu city, during 6 months from January to June 1996. The results was slightly long administration for antibiotics and anti-inflammatory agents, as compared with physiologic healing process after surgery. Conclusively, the author propose that prophylactic antibiotics administration should be limited within 3 to 7 days after operation, and 3 to 5 days for anti-inflammatory drugs.
Assuntos
Humanos , Antibacterianos , Anti-Inflamatórios , Edema , Fraturas Ósseas , Hospitais Gerais , Cirurgia Ortognática , Cirurgia BucalRESUMO
We observed the effect of application of n-PTFE on mandibular bone defects in the rabbit. GTR is based on the hypothesis that during wound healing, non-osteogenic cells are mechanically blocked from entering a bone defect. This allows the slower-migrating mesenchymal cells from the surrounding bone and marrow, having osteogenic potential, to move the defect site. Bilateral through & through defects, 8mmx4mm in size, involving the inferior border of mandible, were created in the mandible angle of 15 rats. The experimental side was applied with high-density n-PTFE membrane, with the opposite side serving as a control. In the first week of the early experimental period, the bone defects of experimental groups were filled with highly vascularized fibrous connective tissue showing prominent osteoblastic activity with osteoid formation, whereas the defects of control were replaced by dense fibro-muscular tissue without osteoblastlf activity. After 3 weeks, the experimental group revealed well formed bone trabeculae and fibro-vascular marrow within surrounding membrane. With time the amount of new bone was decreased with increase of hematopoietic marrow, and the cortical plate composed of dense mature bone was more thicker. In the whole experimental periods, the inflammation and foreign body reaction were not found around the inserted n-PTFE membrane. After removal of membrane there was marked remodeling of newly formed bone by active osteoclasts in periosteal soft tissue, which decreased the bone volume apparently. These findings suggest that n-PTFE has biocompatibility, flexibility, and rigidity and offer rapid bone regeneration within limited area by the membrane. On the other hand, the early removal of membrane caused reduction of bone volume by the osteoclastic resorption. Thus, the time of removal may be more important factor for maintenance of regenerated bone volume.
Assuntos
Animais , Coelhos , Ratos , Medula Óssea , Regeneração Óssea , Tecido Conjuntivo , Reação a Corpo Estranho , Mãos , Inflamação , Mandíbula , Membranas , Osteoblastos , Osteoclastos , Maleabilidade , CicatrizaçãoRESUMO
The author studied on the 197 cases of the pleomorphic adenoma which had been diagnosed with biopsy during the period of 1985 to 1996 at the Kyungpook National University Hospital. This study contains the statistical analysis of the clinico-pathological findings such as sex, age, location, chief complaint, duration, treatment method, size, recurrence, and treatment department in relation to anatomical location. The result were as follows : 1. The treatment department in oxter were ENT(16.2%), GS(22.8%), OMFS(18.8%), PS(12.2%). 2. The incidence of pleomorphic adenoma were parotid gland(57.8%), palatal salivary gland(20.3%), submandibular gland(17.2%), other minor salivary glands(4.5%). 3. The tumors were more common in females than males, with a male to female ratio of 1 : 1.85. 4. The peak age of ocurrence was in the forth decade. 5. The duration of the most cases were less than 5 years. 6. Although the size of the tumors was variable, the incidence was highest between 3cm and 4cm(25.4%). 7. The most prevalent chief complaint was swelling(94.9%). 8. Conservative treatment was performed 30.0%, and radical treatment was employed 70.0%. 9. The recurrence rate is 8.1%, and recurrence used to be relatively common with conservative treatment. 10. The rate of malignant transformation is 1%. 11. The anatomical location has no relationship with the factors of sex, duration, and malignant transformation. 12. There was statistical significance on the anatomical location to the treatment department, age, chief complaint, size, treatment method, recurrence (P<0.05).