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Purpose To investigate the expression of Orai1 and STIM1 in colon cancer and its clinical significance.Methods Immnohistochemistry was used to detect Orai1 and STIM1 protein expression level in 80 cases of colon cancer and 50 cases of normal colon epithelium.The relationships between Orai1 and STIM1 expression and prognosis were statistically analyzed.Result The expression levels of Orai1 and STIM1 in colon cancer were significantly higher than that in normal colon epithelium(P＜ 0.05).Positive expression of Orai1 and STIM1 was correlated with the TNM stage and lymph node metastasis (P ＜ 0.05),but not correlated with gender,age,and differentiation(P ＞0.05).There was a significant positive correlation between Orai1 and STIM1 expression(P =0.001,rs =0.349).Univariate analysis showed that the expression of Orai1 protein,STIM1 protein,TNM stage and lymph node metastasis were significant prognostic factors for colon cancer patients.Conclusion In colon cancer,both Orail and STIM1 proteins may have synergetic functions and promote the development of the tumor,and could be used as a novel biological indicator of anticancer therapy and prognosis.
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Rice bacterial leaf streak,caused by Xanthomonas oryzae pv. oryzicola is a destructive bacterial disease in China. Single-gene resistance to X. oryzae pv. oryzicola has not been found in rice germplasm. A cloned non-host gene from maize with resistance to bacterial leaf streak, Rxo1, was transferred into four Chinese rice varieties through an Agrobacterium-mediated system, including Zhonghua11, 9804, C418 and Minghui86. PCR and Southern analysis of the transgenic plants revealed the integration of the Rxo1 gene into the rice genomes. The integrated Rxo1 was stably inherited, and segregated in a 3:1 (Resistance:Susceptible) ratio in the selfed T1 generations derived from some T0 plants, indicating that Rxo1 inherited as a dominate gene in rice. Transgenic T0 plants and PCR-positive T1 plants were resistant to X. oryzae pv. oryzicola on the basis of artificial inoculation.
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Proteínas de Bactérias , Genética , Metabolismo , Genes de Plantas , Genética , Oryza , Genética , Doenças das Plantas , Genética , Microbiologia , Plantas Geneticamente Modificadas , Genética , Rhizobium , Genética , Transformação Genética , Xanthomonas , Genética , Zea mays , Genética , MicrobiologiaRESUMO
The mutant population of Xanthomonas oryzae pv oryzae strain differential to rice bacterial blight resistance gene Xa23 has been constructed mediated by transposon in vivo . The results of PCR amplification with specific primers and analysis of flanking sequence of mutants indicated that the foreign DNA has been integrated into X. oryzae pv oryzae genome. Four mutants with changed avirulent activity to Xa23 gene have been identified by artificial inoculation. It is possible to clone genes that are required for AvrXa23 avirulence activity using this new strategy.
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Proteínas de Bactérias , Genética , Sequência de Bases , Elementos de DNA Transponíveis , Regulação da Expressão Gênica de Plantas , Genes de Plantas , Dados de Sequência Molecular , Mutação , Oryza , Genética , Microbiologia , Doenças das Plantas , Microbiologia , Plantas Geneticamente Modificadas , Genética , Microbiologia , Virulência , Xanthomonas , Genética , Virulência , FisiologiaRESUMO
Northern corn leaf blight, caused by the fungus Exserohirum turcicum Pass. (Leonard and Suggs), is one of the major diseases in most corn-growing areas of the world. Research on gene tagging of E. turcicum has been limited due to the lack of an efficient transformation system. Since E. turcicum produces and accumulates melamin in cell walls during vegetative growth, it is difficult to efficiently isolate its protoplast. To isolate the protoplast of this pathogen with a high frequency, the effects of cell wall degradation enzymes, including beta-1,3-glucanase (Fungase, Funcelase, Novozyme and Glucanex) and beta-glucuronidase (Driselase, Uskizyme and Kitalase), enzyme concentrations, combinations, strains and medium on the isolation frequency were tested. The isolation frequencies were high enough for transformation when the combinations of (Kitalase + Glucanex + Driselase), (Kitalase + Glucanex) or (Kitalase + Uskizyme) were used. Moreover, the isolation frequencies of protoplast were significantly affected by the cultural morphologies of strain and the growth stage of mycelia. Among the plasmids tested, only plasmid pAN71 is efficient for transformation of E. turcicum. This result will provide some useful information for gene tagging of E. turcicum and other species in Exserohirum.