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Laboratory Animal Research ; : 151-160, 2014.
Artigo em Inglês | WPRIM | ID: wpr-149034

RESUMO

Red ginseng and its extracts have been used as traditional medicines and functional foods in countries worldwide. The aim of this study was to examine the bioavailability of pectin lyase-modified red ginseng extracts (GS-E3D), and the effects of GS-E3D on adipogenesis of 3T3-L1 adipocytes, as well as on metabolic disorders such as hyperglycemia, dyslipidemia, and fatty liver in high-fat diet fed obese C57BL/6 mice. Mice were divided into 5 groups: normal diet group, high fat diet-vehicle group, high fat diet + 0.1 g/kg GS-E3D (0.1-GS-E3D), high fat diet + 0.3 g/kg (0.3-GS-E3D), high fat diet + 1.0 g/kg (1.0-GS-E3D). Treatment of GS-E3D reduced differentiation of 3T3-L1 adipocytes with low cytotoxicity. In the animal model, compared to the high fat diet control, serum glucose, total cholesterol, LDL-cholesterol, HDL-cholesterol, TG, and leptin level were reduced in treatment animals in a dose-dependent manner. In addition, we found that GS-E3D could decrease total hepatic lipid droplets. These results suggest that GS-E3D, as a dietary supplement, has beneficial effects on obesity and may have useful effects in health-care products.


Assuntos
Animais , Camundongos , Adipócitos , Adipogenia , Disponibilidade Biológica , Glicemia , Colesterol , Dieta , Dieta Hiperlipídica , Suplementos Nutricionais , Dislipidemias , Fígado Gorduroso , Alimento Funcional , Hiperglicemia , Leptina , Camundongos Obesos , Modelos Animais , Obesidade , Panax
3.
Journal of the Korean Ophthalmological Society ; : 1847-1852, 2009.
Artigo em Coreano | WPRIM | ID: wpr-96508

RESUMO

PURPOSE: To evaluate the clinical reliability of Pentacam(R) by comparing anterior chamber parameters measured by Oculus Pentacam system (Oculus Inc., Germany) and Hi-scan ultrasound biomicroscopy (OPTIKON 2000, Rome, Italy) in primary angle closure (PAC) and normal patients. METHODS: A prospective study was performed from June 2006 to January 2007. Fifty-one eyes in 26 primary angle-closure patients and 39 eyes in 20 normal control patients, for a total of 90 eyes of 46 patients were recruited from glaucoma out-patient clinics. The correlation and agreement of both measurements of anterior chamber depth and anterior chamber angle measured by UBM and Pentacam(R) were calculated. Sensitivity and specificity of each tool were also compared and the diagnostic value of angle closure was examined. RESULTS: Anterior chamber depth measured by UBM and Pentacam(R) showed strong correlation in the normal control group (r=0.821) and PAC group (r=0.957). Anterior chamber angle showed moderate correlation in the normal control group (r=0.523) and PAC group (r=0.456) while good agreement was also observed. In diagnosing angle closure, anterior chamber measurements appear similar in UBM and Pentacam(R) using the ROC curve (AUC of UBM, 0.942; AUC of Pentacam(R), 0.931). CONCLUSIONS: Pentacam(R) shows high to moderate correlation of anterior chamber measurements and good agreement compared with UBM. Pentacam(R) shows similar ability in diagnosing angle closure compared with UBM and thus is expected to be a good tool in diagnosing angle closure.


Assuntos
Humanos , Câmara Anterior , Área Sob a Curva , Olho , Glaucoma , Microscopia Acústica , Pacientes Ambulatoriais , Estudos Prospectivos , Curva ROC , Cidade de Roma , Sensibilidade e Especificidade
4.
Korean Journal of Ophthalmology ; : 143-144, 2008.
Artigo em Inglês | WPRIM | ID: wpr-67678

RESUMO

To report a case of cytomegalovirus (CMV) retinitis after intravitreal injection of triamcinolone acetonide (IVTA). A 77-year-old woman with macular edema due to central retinal vein occlusion (CRVO) developed peripheral retinitis 4 months after IVTA. A diagnostic anterior chamber paracentesis was performed to obtain DNA for a polymerase chain reaction (PCR) test for viral retinitis. The PCR test was positive for CMV DNA. Other tests for infective uveitis and immune competence were negative. Four months after presentation, gancyclovir was intravitreously injected a total of 5 times, and the retinitis resolved completely. CMV retinitis is a rare complication of local immunosuppression with IVTA. It can be managed with timely injection of intravitreal gancyclovir until recovery from local immunosuppression.


Assuntos
Idoso , Feminino , Humanos , Antivirais/uso terapêutico , Citomegalovirus/genética , Retinite por Citomegalovirus/diagnóstico , DNA Viral/análise , Ganciclovir/uso terapêutico , Imunossupressores/efeitos adversos , Injeções , Edema Macular/tratamento farmacológico , Reação em Cadeia da Polimerase , Oclusão da Veia Retiniana/complicações , Triancinolona Acetonida/efeitos adversos , Corpo Vítreo
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