Blended Teaching Practice of Molecular Biology Driven by Discipline Frontiers / 中国生物化学与分子生物学报

Molecular Biology is the core course of biology major, whose fundamentals are classical and which develops rapidly as well. The introduction of the discipline frontiers is an ideal way to enhance the vitality of the classroom. Based on MOOC resources, the course group combined online independent study and offline face to face class to implement blended learning. Driven by the discipline frontiers, diversified teaching methods were introduced and classroom ecology was reconstructed. Multiple teaching sessions, including students’ independent study, questioning and presentation, class leading role of the teachers, questions and answers and knowledge expansion have been rolled out and unified so as to cultivate the science and humanity spirit of the students and enhance the vitality of the classroom. This blended teaching model effectively exercises students’ innovative thinking, improves students’ participation, and plays a positive role in enriching learning experience, stimulating professional dream and promoting students’ enlightenment.

Transcriptome profiling and analysis of Panax japonicus var. major / 中国中药杂志

The rhizome of Panax japonicus var. major have been used as the natural medicinal agent by Chinese traditional doctors for more than thousand years. Most of the therapeutic effects of P. japonicus var. major had been reported due to the presence of tetracyclic or pentacyclic triterpene saponins. In this study, Illumina pair-end RNA-sequencing and de novo splicing were done in order to understand the pathway of triterpenoid saponins in this species. The valid reads data of 15. 6 Gb were obtained. The 62 240 unigenes were finally obtained by de novo splicing. After annotation, we discovered 19 unigenes involved in ginsenoside backbone biosynthesis. Additionally, 69 unigenes and 18 unigenes were predicted to have potential function of cytochrome P450 and UDP-glycosyltransferase based on the annotation results, which may encode enzymes responsible for ginsenoside backbone modification. This study provides global expressed datas for P. japonicus var. major, which will contribute significantly to further genome-wide research and analysis for this species.