Effects of cilostazol on LPS-stimulated adhesion and soluble adherent molecules release / 中国药理学通报

AIM To examine the effect of cilostazol, a no vel selective phosphodiesterase type 3 inhibitor, on adherence between neutrophils and human umbilical e ndothelial cells ( HUVECs ) and investigate its possible mechanisms. MET HODS Confluent HUVECs between 4～6 passages were used and stimulated by l ipopolysaccharide (LPS, 5 mg?L -1 ) with or without coincubation of cilosta zol (1～10 ?mol?L -1 ) for 24 h. Soluble cell adhesion molecules (sCAMs), including vascular cell adhesion molecule-1 (sVCAM-1), intercellular adhesion molecule-1 (sICAM-1) and endothelial leukocyte adhesion molecule-1 (sELAM-1, sE-selectin) in cell culture medium were measured by ELISA. RESULTS Cilostazol (1～10 ?mol?L -1 ) inhibited adherence between neutrophils and HUVECs in a dose- dependent manor. At the same time, cilostazol didn't affect sICAM-1 and sE-sel ectin release from LPS-stimulated HUVECs, but in contrast, it significantly inh ibited sVCAM-1 production under the same experiment condition, and this effect was canceled by H-89, an inhibitor of protein kinase A ( PKA ). CONCLUS ION Cilostazol significantly inhibits adherence between neutrophils and H UVECs, and downregulates sVCAM-1 release from LPS-activated HUVECs, and these effects on cytokine-challenged endothelial cells might be via a PKA-dependent pathway. The present result suggests that cilostazol partially eliminates some o f the adherent reactions of HUVECs to LPS, a deleterious cytokine, and it is rea sonable to consider that cilostazol might be a strategy for preventing atheroscl erosis and other cardiovascular diseases.

Metabolites of marine fungus Julella avicenniae from South China Sea(Ⅱ) / 中草药

Objective To stuty the metabolites of marine fungus Julella avicenniae from the South China Sea.Methods The fungus J.avicenniae was extracted with AcOEt,separated,and purified by column chromatographies on silica gel.All the compounds were identified on the basis of spectral analysis(IR,MS,NMR) and chemical evidence.Results One novel compound was identified from its extract of marine fungus J.avicenniae from the South China Sea.Conclusion One novel compound,avicenniaene,is isolated from the marine fungus J.avicenniae from the South China Sea.

ADVANCES IN STUDIES OF SUBERGORGIC ACID / 中国海洋药物

Subergorgic acid is one of marine neurotoxins. It has a unique chemical structure. This article reviews its isolation and structural determination, total synthesis,derivatives preparation,biologic activities and potential applications.

SIX KINDS OF N-ACYLSPHINGOSINES FROM THE CHINESE GORGONIA JUNCEELLA SQUAMATA / 中国海洋药物

Six N - acylsphingosines with molecular weight 509,537,565,607 and 621 respectively were isolated from the Chinese gorgonia Junceella squamata . The chain length of the carboxylic acid in them were C14,C16,C18,C20,C21 and C22,and the chain length of the bases were all C18. On the basis of their spectra and GCMS of the Chemical reaction products,their structure were elucidated.

EFFECT OF RAPESEED PEPTIDES ON GROWTH OF SARCOMA_(180) CELL INCUBATED IN VITRO / 营养学报

Objective: To study the effect of rapeseed peptides (RSP) from enzymolysis of rapeseed protein on growth of Sarcoma 180 (S180) cell incubated in vitro and its cell membrane. Methods: S180 cell incubated in vitro was used as model. The change of proliferation of S180 cell, fatty acid and sialic acid in membrane and membrane fluidity of S180 cell were investigated. Results: RSP had inhibitiory ability to S180 cell proliferation and could influence its membrane. Conclusion: RSP had potential in inhibiting tumor growth, so was needed to study further.