RESUMO
Objective:To investigate the effect of HOXa-10 gene in the mouse uterus during embryo implantation. Method: real-time fluorescence quantitative PCR (FQ-PCR) and immunohistochemistry were used to detect the HOXa-10 gene and protein of the mouse of un-pregnant pregnant d1,d2,d3,d4,d5 and d7. Result:The HOXa-10 mRNA/?-actin mRNA expression in pregnant group is higher than that of non-pregnant grnup, and the results showed gradual hoist trend as days past, the HOXa-10 mRNA expression reached the maximum at pregnant d4. Immunohistochemical analysis showed the same results as FQ-PCR. Conclusion:HOXa-10 is persistently expressed in mouse endometrium during the early pregnancy and maybe participates in the regulation process of mouse blastodyst implantation.
RESUMO
Objective To investigate the effect of PTEN gene in the mouse uterus during embryo implantation.Methods Real-time fluorescence quantitative PCR(FQ-PCR) was used to detect PTEN mRNA expressed in the endometria of the nonpregnant mice and the late pregnant mice(day 1,day 3,day 4,day 5 and day 7),with 20 mice sacrificed at each fixed day.Out of another 20 3-day pregnant mice,ten received PTEN antisense oligonucleotide at the horn of uterus and ten received normal saline to count the blastocysts at pregnant day 8.Results The PTEN mRNA/?-actin mRNA in pregnant mice was higher than that of nonpregnant mice,gradually hoisted as days passed by,and reached the highest at pregnant day 5.The number of blastocysts in the mice that received PTEN antisense oligonucleotide was fewer than that received normal saline.Conclusion PTEN persistently expresses in mouse endometria during the early pregnancy and maybe participate in the regulation process of mouse blastodyst implantation.