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Objectives To investigate the dynamic change of galectin-1 in myocardium of mice with viral myocarditis (VMC). Methods A total of 58 male BALB/c mice were selected and randomly divided into CVB3 group (n=50) and control group (n=8). Mice in CVB3 group were infected with 0.10 ml 10-5/L CVB3 through intraperitoneal inoculation, and 8 mice were killed on day 7, 10, 14 and 28 respectively after inoculation. Mice in control group inoculated with 0.10 ml eagle reagent was killed on day 28. The myocardial pathological changes were observed using light microscope. In addition, expressions of serum galectin-1 were detected by ELISA and expressions of myocardial galectin-1 were detected by real-time quantitative-polymerase chain reaction (RQ-PCR). Results Compared with control group, the myocardial expression of galectin-1 mRNA in CVB3 group was obviously increased on day 7 and day 10 (all P<0.05);the serum concentration of galectin-1 in CVB3 group was obviously increased on day 7, day 10 and day 14 (all P<0.05). Conclusion The expressions of galectin-1 in myocardium of mice with viral myocarditis were in dynamic changes. Galectin-1 may play a role in the pathogenesis and development of viral myocarditis.
RESUMO
AIM: To observe the effect of ligustrazine on the calcium contents and calmodulin activity of lung tissue in rats of the bleomycin-induced pulmonary fibrosis. METHODS: Thirty-two SD rats were replicated into pulmonary fibrosis models by intratracheal injection of bleomycin A 5 in one lump and were divided into two groups randomly, untreated model group (n=16) and ligustrazine group(n=16). The calcium contents and calmodulin activity of the lung of the rats of each group were assayed on d 14, d 28 after the injection and compared with control group (n=8). RESULTS: The calcium contents and calmodulin activity of model group were obviously higher than control group (P