Expression of c-Met and c-Src in non-small cell lung cancer and its relationship with prognosis / 中国医师进修杂志

Objective To explore the expressions of c-Met and c-Src in non-small cell lung cancer (NSCLC), and its relationship with clinical pathological characters and prognosis. Methods The c-Met and c-Src expressions were detected by immunohistochemistry in 88 patients with NSCLC from April 2011 to January 2013. The relationship between the expressions of c-Met and c-Src and clinical pathological features and prognosis were analyzed. Results The c-Met and c-Src were all significantly expressed in NSCLC tissues, and no expression showed in interstitial and normal lung tissues. The expressions of c-Met and c-Src in patients with NSCLC were associated with sex, differentiation, pathology type, T staging and TNM staging (P<0.05 or <0.01); and the expression of c-Met was associated with lymph node metastasis (P<0.01). The expressions of c-Met and c-Src in patients with NSCLC were not associated with age, and the expression of c-Src was not associated with lymph node metastasis (P>0.05). Pearson correlation analysis result showed that the expressions of c-Met and c-Src in lung cancer tissues was positive correlation (r=0.662, P<0.01). Kaplan-Meier survival curve analysis result showed that the disease free survival time (DFS) and overall survival time (OS) in c-Met high expression patients (51 cases) were significantly shorter than those in c-Met low expression patients (37 cases): (18.08 ± 1.34) months vs. (23.76 ± 1.79) months and (33.63 ± 1.95) months vs. (42.24 ± 2.68) months, the DFS and OS in c-Src high expression patients (25 cases) were significantly shorter than those in c-Src low expression patients (63 cases): (16.96 ± 2.56) months vs. (21.86 ± 1.15) months and (27.84 ± 2.89) months vs. (40.98 ± 1.81) months, the DFS and OS in both c-Met and c-Src high expression patients (25 cases) were significantly shorter than those in both c-Met and c-Src low expression patients (37 cases): (16.96 ± 2.56) months vs. (23.76 ± 1.79) months and (27.84 ± 2.89) months vs. (42.24 ± 2.68) months, and there were statistical differences (P<0.05). Cox multiplicity result showed that T staging (RR=2.174, 95％CI 1.354 to 3.490, P=0.001) and high expressions of c-Met and c-Src (RR=1.447, 95％CI 1.114 to 1.880, P=0.006) were the independent risk factors of DFS in patients with NSCLC;pathology type (RR=0.610, 95％CI 0.377 to 0.986, P=0.044), T staging (RR=2.215, 95％CI 1.357 to 3.616, P=0.001) and high expressions of c-Met and c-Src (RR=1.979, 95％CI 1.455 to 2.692, P = 0.000) were the independent risk factors of OS in patients with NSCLC. Conclusions The c-Met and c-Src are involved in the development of NSCLC and affect the prognosis of patients with NSCLC.

Progress of long non-coding RNA in non-small cell lung cancer / 中国医师进修杂志

Lung cancer is the leading cause of cancer-related deaths worldwide, among which more than half of non-small cell lung cancer had been locally advanced or distant metastasis at the time of diagnosis.Therefore,it has a poor prognosis.In recent years,long non-coding RNA(lncRNA)has drawn great attention in the field of oncology.Abnormally expression of lncRNA plays an important role in the development of non-small cell lung cancer through different transcriptional, post-transcriptional or epigenetic mechanisms.This review summarizes the current literature on lncRNA in non-small cell lung cancer, introduces the functions and influences of lncRNA in the biology of non-small cell lung cancer and highlights its clinical potential value as a tumor biomarker and potential therapeutic target.

Gastroesophageal reflux disease is associated with high risk of obstructive sleep apnea syndrome / 中华内科杂志

Objective To investigate the relationship between gastroesophageal reflux disease (GERD) and obstructive sleep apnea syndrome(OSAS). Methods Patients diagnosed as GERD and healthy controls without GERD related symptoms or endoscopic esophagitis were enrolled from October 2017 and December 2017. All subjects completed Berlin Questionaire to assess the risk of OSAS. Univariate and multivariate logistic regression models were applied to identify risk factors of OSAS. Results A total of 177 subjects (97 GERD, 80 controls) were finally selected. Significantly more patients in GERD group had high risk OSAS than those in controls [36.1％(35/97) vs. 17.5％(14/80), P=0.005]. In GERD group, patients with erosive reflux diseases (ERD) had especially higher proportion of high risk OSAS compared with the non-ERD group and the healthy controls [53.3％(24/45) vs. 20.8％(10/48) and 17.5％(14/80), P=0.001]. On univariate analysis, male, aging and reflux esophagitis were identified as risk factors of OSAS (all P<0.01). On multivariate analysis, male (OR=12.156, 95％CI 1.382-106.905, P=0.024), aging (OR=1.132, 95％CI 1.051-1.220, P=0.001), acid regurgitation with reflux esophagitis (OR=5.157, 95％CI 1.327-20.034, P=0.018) were significant risk factors. Conclusions More GERD patients are combined with high risk OSAS than controls, especially subjects with reflux esophagitis. Male and aging GERD patients with acid regurgitation and reflux esophagitis need further evaluation on OSAS screening.

Research progress on mechanism of low-dose chemotherapy / 实用肿瘤学杂志

Chemotherapy is one of the main ways for comprehensive treatment of tumors,and has played an important role in tumor treatment for many years. However,in recent years,low-dose chemotherapy( Low-dose chemotherapy) has gradually become a clinical treatment strategy commonly used to taken a reasonable mode of administration and planning,relative to the maximum tolerated dose of chemotherapy with small side effects,pa-tient tolerance significantly improved,better efficacy and other. In this paper,the mechanism of low-dose chemo-therapy on the progress is reviewed systematicly.

Clinical Significance of FGFR1OP and p57 (Kip2) Proteins in Gliomas: An Immunohistochemical Study / 中国肿瘤临床

Objective: To investigate the effects of FGFR1OP and p57/Kip2 proteins on the genesis and progression of gliomas and their clinical significance. Methods: The expression of FGFR1OP and p57/Kip2 in 54 glioma specimens was detected by SP immunohistochemical technique. The relationship between the ex-pression levels of those proteins and various clinical pathologic factors was evaluated. Results: The expres-sion of FGFR1OP and p57/Kip2 was found in 66.7% and 44.4% gliomas, respectively. The OD value of FG-FR1OP was 0.131±0.010 in high grade gliomas, and 0.118±0.010 in low grade ones, with a statistical signifi-cance (t=-5.497, P=0.000), showing that higher expression of FGFR1OP was significantly associated with glo-ma cell differentiation. The OD value of p57/Kip2 was 0.156±0.008 in high grade gliomas, and 0.165±0.006 in low grade ones, with a statistical significance (t=0.296, P=0.014), showing that lower p57/Kip2 expression was correlated with high grade gliomas. FGFR1OP was negatively correlated with p57/Kip2 in gliomas (r=-0.732, P<0.01). Conclusion: Increased expression of FGFR1OP and/or decreased expression of p57/Kip2 may play an important role in the genesis and progression of gliomas and may indicate a poor prognosis.

Effect of X-irradiation on the protein expression of P57kip2 and TGF-β1 in lung cancer cell stain A549 / 中华放射医学与防护杂志

Objective To analyze the effect of X-irradiation on the proteins expression of p57kip2 and TGF-β1 in lung cancer cell stain A549 and its clinical significance.Methods Lung cancer cell stain A549 was cultivated and cell,protein was extracted at 6,12,24,36 and 48 hours after X-irradiation by differenl doses(2,4, 8 and 12 Gy).The expression of p57kip2 and TGF-β1 proteins were examined by Western blot.Results The expression of p57kip2 in lung cancer cell stain A549 was very low before X-irradiation.and increased significantly after irradiation with difierent doses and reached the peak level at 12 hours after irradiation(P<0.05).TGF-β1 reached its peak 1evel at 6 hours after irradiation(P<0.05).Conclusions X-irradiation can up-regulate the expression of p57kip2 and TGF-β1 proteins which increased with certain doses.p57kip2 and TGF-β1 could be usedto predict the damage degree of cancer cells by X-ray.

Effect of Jian-wei decoction on the quality of ulcer healing and expression of EGFRmRNA in the rat model of gastric ulcer / 中国基层医药

Objective To investigate the the effect and mechanism of Jian-wei decoction(JWD) on quality of ulcer healing.Methods The rat models of chronic gastric ulcer were induced by acetic acid,the effects of QOUH of JWD on the model of gastric ulcer were observed,the expression of EGFRmRNA of mucosa were measured in situ hybridization.Results JWD could improve the injury of gastric mucosa and increase the expression of EGFRmRNA in the tissue around peptic ulcer(PU).Conclusion JWD increases the expression of EGFRmRNA in the tissue around PU which might be one of possible mechanisms that JWD improves quality of ulcer healing.