RESUMO
[Objective]To explore the effects of invigorating the kidney,promoting qi and activating blood method in repairing articular cartilage defects in rabbits with chodrocytes induced by bone marrow matrix stem cell.[Method]The complexes from seeding of chondrocytes and allograft chondrocytes encapsulated in collagen gel and cultured in Guyanding drug serum into CPPf/PLLA scaffolds were cultured in vitro for 3 weeks. Some complexes were observed with the phase-contrast microscope or scanning electron microscope and others were allotransplanted into articular cartilage defects in rabbits.The specimens harvested at 4,8,12 weeks were analyzed by observation,histology and type Ⅱ collagen immunohistochemistry.[Result]The induced chondrocytes were embedded in scaffolds evenly the formation,smoothness of the surface and integration with adjacent tissues of the hyaline cartilage-like tissues,distribution of type Ⅱ collagen in the matrix of the induced chondrocytes group were better than all those in the chondrocyte group.[Conclusion]The method of invigorating the kidney,promoting qi and activating blood has superiority in repairing articular cartilage defects over the traditional method.