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Objective To carry out a meta-analysis,in order to evaluate the effectiveness and safety of the duodenum-preserving pancreatic head resection (DPPHR) and pancreaticoduodenectomy (PD) or pylorus-preserving pancreaticoduodenectomy(PPPD) for surgical treatment of chronic pancreatitis.Methods Medline,EMBASE,Cochrane library and other medical databases were searched for the clinical trials (randomized controlled trials) of comparing DPPHR Versus PD/PPPD.A total of 5 clinical trials (8 references) met the inclusion criteria.The data were analyzed using the RevMan 5.3 software.Results The two methods don't have statistical differ ence in terms of operation time (P=0.007),postoperative morbidity (P=0.35) and mortality (P=0.18),pain relief(P=0.36),new onset of diabetes(P=0.11),exocrine insufficiency(P=0.18),short-term(P=0.14) and long-term(P=0.16) quality of life score,the length of hospital stay (P=0.69),and pancreatic fistula (P=0.78).Weight gain (P＜0.000 01) and occupational rehabilitation (P=0.03)were significantly improved in the DPPHR group.However,PD/PPPD group was associated with fewer readmission due to pancreatic diseases.Conclusions DPPHR offers more advantages with regard to the quality of life.However,it needs more high-quality clinical trials to verify the results.
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<p><b>OBJECTIVE</b>To explore the clinical and genetic characteristics of a case with Pallister-Killian syndrome (PKS).</p><p><b>METHODS</b>Chromosomal karyotype of umbilical cord blood sample derived from a 36-year-old pregnant woman was analyzed by G-banding analysis. After birth, the child was further analyzed with single nucleotide polymorphism microarray (SNP array) and fluorescence in situ hybridization (FISH) using 12pter/12qter probes.</p><p><b>RESULTS</b>G-banding analysis showed that the fetus has a karyotype of 46,XY [77]/47,XY,+mar [23]. After birth, Affymetrix CytoScan 750K array analysis showed a segmental tetrasomy of arr [hg19] 12p13.33p11.1(173 786 - 34 835 641)×4 and a 34.6 Mb repeat at 12p13.33p11.1 with in the neonate. FISH analysis confirmed that 39% of cells harbored the 12p tetrasomy.</p><p><b>CONCLUSION</b>Combined clinical examination, G-banded chromosomal karyotyping, FISH and microarray analysis can delineate the origin and fragments of small supernumerary marker chromosomes and diagnose PKS with precision.</p>
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Adulto , Feminino , Humanos , Gravidez , Bandeamento Cromossômico , Transtornos Cromossômicos , Diagnóstico , Cromossomos Humanos Par 12 , Hibridização in Situ Fluorescente , Métodos , Cariotipagem , Análise de Sequência com Séries de Oligonucleotídeos , Polimorfismo de Nucleotídeo Único , Diagnóstico Pré-Natal , MétodosRESUMO
Objective To establish the pyrogen removing effect of activated carbon in the technics of traditional Chinese medicine (TCM) injections. Methods The content of bacterial endotoxin concentration was detected by kinetic turdimetric assay to evaluate the effect of removing pyrogen before and after using activated carbon in concentrated solution of TCM injection. Results The activated carbon adsorption rate of Shuanghuanglian concentrated solution≥70%and the activated carbon adsorption rate of Danshen concentrated solution>95%. Conclusion Pyrogen manufacturing process is scientific and rational by adding activated carbon adsorption in powder injection of TCM. The bacterial endotoxin of large doses can't be removed fully by activated carbon adsorption.
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Objective To optimize the extraction process of Yangwei Jinshi Granules. Methods An orthogonal test was adopted in the study. With the paste-forming rate, the content of extract and the content of hesperidin of Yangwei Jinshi Granules as indices, data processing was carried out with grading method of synthesizing multiple indexes for optimizing the extraction condition. Results The best extraction technology was as follows:boiled 2 times, first with 10 times amount of water for 1.5 h, and then boiled with 8 times amount of water for 1 h. Conclusion The optimized process is reasonable, stable and economic for Yangwei Jinshi Granules.
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AIM: To establish a HPLC method for simultaneously determining chlorogenic acid,caffeic acid,forsythin,forsythoside A and baicalin in Shuanghuanglian Powder for Injection (Flos Lonicerae japonicae,Fructus Forsythiae,Radix Scutellariae,etc.).METHODS: HPLC assay was carried out on a Phenomenex~((R)) Luna C_(18) column (250 mm×4.6 mm,5 μm).The mobile phase was methanol-0.2% phosphoric acid solution as gradient elution.The flow rate was 1.0 mL/min.The detection wavelength was set as multi-wavelength detection.RESULTS: The linear ranges of chlorogenic acid,caffeic acid,forsythin,forsythoside A and baicalin fell within the range of 0.204-2.04 μg ,0.010 2-0.102 μg,0.186-1.86 μg,0.058 5-0.585 μg and 2.52-25.2 μg,respectively.The five components showed good linear correlations ( r = 0.999 7,0.999 4,0.999 8,0.999 8,0.999 9 ).The average recoveries of five components were in accordance with the determination requirements.CONCLUSION: The method is simple,quick,reliable,accurate and very suitable to the quality control of Shuanghuanglian Powder for Injection.
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Objective To establish a determination method of Allicin in Garlic oil Sustained-release Tablets. Method Diamonsil C18 column was used with a mobile phase of mathanol-water-formic acid (80∶20∶0.1). The detective wavelength was at 218 nm. The flow rate was 1.0 mL/min. Column temperature was at 25 ℃. Results Allicin showed a good linear relationship at the range of 0.05～0.25 ?g (r=0.999 9). The average recovery rate was 99.5% (RSD=1.7%). Conclusion The method is simple, accurate, highly sensitive and reproducible. It can be used for the quantitative determination of Allicin in Garlic oil Sustained-release Tablets.
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Objective To establish a method of HPLC gradient elution method determination of Notoginsenoside R1, Ginsenoside Rg1 and Rb1 in Naodesheng Capsules. Methods Kromasil C18 column was used with a mobile phase of acetonitrile-water gradient elution, column temperature at 25 ℃. Results Notoginsenoside R1, Ginsenoside Rb1 and Ginsenoside Rg1 showed good linear relationship at the range of 0.495～1.782 ?g (r =0.9999), 2.65～9.54 ?g (r =0.9995), 1.85～6.66 ?g (r =0.9999), respectively. The average recovery:Notoginsenoside R1 was 100.60% (RSD=1.99%), Ginsenoside Rb1 was 100.86% (RSD=2.19%) and Ginsenoside Rg1 was 98.83% (RSD=1.95%), respectively. Conclusion The methods is simple, accurate, highly sensitive and reproducible. It can be used for the quantitative determination of Notoginsenoside R1, Ginsenoside Rg1 and Rb1 in Naodesheng Capsules.
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Objective To determine the total sodium in Danshen for injection.Methods Flame atomic absorption spectroscopy was used to determine the total sodium in Danshen for injection.Results The calibration curves were linear in the range of 0.5～10 mg/L for sodium chloride.The average recovery rates of sodium chloride were 101.8%,RSD=1.60%.Conclusion This method was found to be simple,quick and accurate for simultaneous determination of sodium in Danshen for injection.
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Objective To optimize the formulation of the Garlic oil ?-CD sustained-release tablet. Methods The preparation formulation of the sustained-release tablet was optimized by orthogonal design which took the released rate as the index. Result The optimized formulation contained 9% HPMC, 15% lactose, 10% PVP, 10% microcrystalline cellulose. The ingredient of Garlic oil sustained-release tablet by this formulation was released slowly in 8 h. Conclusion The optimized formulation is rational and stable, the tablet could be released slowly.
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AIM:To establish a HPLC method for simultaneously determining chlorogenic acid,caffeic acid,forsythin,forsythoside A and baicalin in Shuanghuanglian Powder for Injection(Flos Lonicerae japonicae,Fructus Forsythiae,Radix Scutellariae,etc.).METHODS:HPLC assay was carried out on a Phenomenex Luna C_ 18 column(250 mm?4.6 mm,5 ?m).The mobile phase was methanol-0.2% phosphoric acid solution as gradient elution.The flow rate was 1.0 mL/min.The detection wavelength was set as multi-wavelength detection.RESULTS:The linear ranges of chlorogenic acid,caffeic acid,forsythin,forsythoside A and baicalin fell within the range of 0.204-2.04 ?g、0.010 2-0.102 ?g、0.186-1.86 ?g、0.058 5-0.585 ?g and 2.52-25.2 ?g,respectively.The five components showed good linear correlations(r=0.999 7、0.999 4、0.999 8、0.999 8、0.999 9).The average recoveries of five components were in accordance with the determination requirements.CONCLUSION:The method is simple,quick,reliable,accurate and very suitable to the quality control of Shuanghuanglian Powder for Injection.