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1.
Chinese Journal of Zoonoses ; (12): 987-989,996, 2014.
Artigo em Chinês | WPRIM | ID: wpr-602027

RESUMO

Interactions between FMDV and cardiac cells are multifaceted and complex ,these interactions leads to pro-teins alterations in cardiac cells inevitably .To understand the pathogenesis of myocarditis after FMDV infection in mice ,the suckling mouse model for myocarditis induced by foot-and-mouth disease virus (FMDV) was established in this study .Suckling mice within 3 days old was selected to infect by FMDV .Myocarditis caused by FMDV in suckling mice was confirmed with clinical symptom monitor .The observation of Hematoxylin and eosin stain (H&E stain) and transmission electron microscopy (TEM) were performed after samples processing .According to conventional polymerase chain reaction (PCR) methods ,prim-ers of VP1 gene was designed ,synthesised and specific FMDV VP1 gene was amplified from the heart muscle of suckling mice . The results indicated that suckling mice appeared low spirit condition ,dyspnea ,and dull reaction within 36 hours after chal-lenge with FMDV .Infiltration of inflammatory cells and dissolution of myocardial fibers were observed with H&E stain and TEM .Special target gene of FMDV was amplified from the heart of infected group .Obvious inflammation in the heart of suck-ling mice caused by FMDV was observed .It's suggested that suckling mouse model for myocarditis induced by FMDV was es-tablished successfully ,which would lay the foundation for researches of myocarditis mechanism in young cloven-hoofed ani-mals .

2.
International Journal of Laboratory Medicine ; (12): 1285-1286, 2014.
Artigo em Chinês | WPRIM | ID: wpr-446556

RESUMO

Objective To investigate the diagnostic value of human leukocyte antigen (HLA )-B27 for ankylosing spondylitis (AS) .Methods 2 768 patients with suspected AS were enrolled ,including 600 patients with confirmed AS .Flow cytometry was performed to detect blood HLA-B27 .Results Among 2 768 patients with suspected AS ,598(21 .6% ) patients were found HLA-B27 positive and 2 170(78 .4% ) patients negative .Difference of HLA-B27 positive rates between male and female patients was sta-tistically significant(P< 0 .05) .HLA-B27 positive rate of patients aged 20 - < 40 years was significantly higher than the others (P<0 .05) .Among 600 patients with confirmed AS ,536 cases(89 .3% ) was found HLA-B27 positive .Conclusion HLA-B27 de-tection combined with clinical manifestations contribute to early diagnosis of AS .

3.
Chinese Journal of Rehabilitation Theory and Practice ; (12): 96-98, 2005.
Artigo em Chinês | WPRIM | ID: wpr-977955

RESUMO

@# ObjectiveTo study the effect and mechanisms of nourishing Piyin Remedy (nPR) and bovine brain extract (bBE) on experimental spinal cord injury (SCI) of rat.Methods80 healthy SD rats were divided into 5 equal groups randomly: bBE group supplied through subarachnoid cavity, normal saline (NS) group supplied through subarachnoid cavity, nPR group, NS orally taken group, combined group. Animal models were made by Allen's equipment on T8~T9 segment. The spinal nerve function, somatosensory evoked potentials (SEP), retrograde and label technique of horseradish peroxidase, gross observation, histological and morphometric analysis were taken as the observed indices.ResultsThe values of observed indices of bBE group and nPR group improved evidently compared with their own control groups; that of combined group was prior to sole administration.ConclusionnPR can hold back the secondary SCI and accelerate the recovery of spinal nerve function; bBE can stimulate the improvement of injuried nerve fibers; the joint of nPR and bBE can make a synergic effect.

4.
Journal of Xi'an Jiaotong University(Medical Sciences) ; (6)2004.
Artigo em Chinês | WPRIM | ID: wpr-548134

RESUMO

Objective To detect the expression of AKT2 in human colon cancer and understand its relationship with PTEN.Methods The expressions of AKT2 and PTEN were detected in 30 patients with normal colonic tissues,30 patients with colon adenoma tissues,and 64 patients with colon carcinoma tissues by immunohistochemical SP staining method.Results The positive expressive rate of PTEN presented a trend of progressive decrease from normal tissues,adenoma tissues to colon carcinoma tissues,while the positive expression rate of AKT2 presented a trend of progressive increase.PTEN expression was obviously higher in normal colon tissues than in colon adenoma tissues and colon cancer tissues(?2=68.855,P

5.
Journal of Practical Radiology ; (12)2001.
Artigo em Chinês | WPRIM | ID: wpr-539729

RESUMO

Objective To discuss CT appearances and diagnosistic value of invasive thymoma.Methods CT findings in 13 cases of invasive thymoma proved by surgery and pathology were retrospectively analysed. Results In 13 cases, invasive thymomas all showed as solf tissue masses in anterier mediastinum on CT, of them, 10 cases were of heterogeneous density and irregular invasion to adjacent organs which the main CT findings were pleural implants in 3, cardiovascular involvement in 9 and abdominal invasion in 1 were found in 11 cases. Conclusion Conventional CT scan plays an important role in diagnosis and differential diagnosis of invasive thymoma.

6.
Chinese Journal of Parasitology and Parasitic Diseases ; (6)1997.
Artigo em Chinês | WPRIM | ID: wpr-684054

RESUMO

Objective To systematically study the development and morphological changes of Taenia solium cysticerci in the period from the appearance to maturation. Methods Pigs were orally infected with T.solium eggs. Autopsies were made periodically to get tissue specimens and examine the distribution of cysticerci. Cysticerci were excised, pressed between two microscopic slides for microscopical observation. Sections were also made for histological study. Results Cysticerci distributed mainly in muscles, secondly in brain, liver, lung and kidney. The size of cysticerci was highly variable at different parts or even in the same part of the same host. The early stage cysticercus was observed on the 19th day without suckers and hooklets on the scolex region, and was only found in skeletal muscles. Histological examination revealed that early development of the scolex became obvious. No cysticerci were found in other tissues. At 30 d, the cysticerci possessed hooklets and brood suckers. With a longer duration of infection, the diameter of suckers and length of hooklets both increased. Larger suckers and more folds on scolex appeared 60 d later. Morphologically, cysticerci after 60 d were similar to those found at 60 d. Degenerated cysticerci were found at every stage. Conclusion Cysticerci appear in about 19 days after the pig was infected with eggs of T.solium and the duration needed for maturation is about 60 d.

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