RESUMO
Objective To explore the clinical effect of postoperative early enteral nutrition in the elderly patients with total gastrectomy. Methods Fifty seven elderly patients with total gastrectomy were divided into postoperative routine nutrition(PRN), postoperative early parenteral nutrition(PEPN), and postoperative early enteral nutrition(PEEN) groups. The levels of serum albumin(ALB), transferrin(TF), pre-albumin(PA), CD3, CD4, CD8, CD4/CD8 were detected 1 day before(PRED 1 ) and 9 days after(POD 9 ) operation, and the postoperative infectious complications were observed , and the length of hospital stay(LOS) and the cost of nutritional support were determined. Results The levels of ALB, TF, PA, CD3, CD4 and CD4/CD8 on POD 9 were (34.3?3.5)g/L, (1.7?0.2)g/L, (258.8?20.2)mg/L,(39.7?5.4)%,(34.3?4.0)% and (1.5?0.3) in PEPN as well as (33.9?3.1)g/L, (1.7?0.2)g/L, (260.5?24.7)mg/L, (42.8?6.5)%, (37.2?4.5)% and (1.7?0.2) respectively, which were all significantly lower than (29.5?3.0)g/L, (1.3?0.2)g/L, (235.4?13.9)mg/L, (34.8?5.0)%, (30.4?4.4)% and (1.2?0.2) in RN group (all P
RESUMO
Objective To study the expression of RhoC mRNA in human primary hepatocellular carcinoma(PHCC) and paracarcinoma liver(PCL) tissues .Method Reverse transcription polymerase chain reaction(RT-PCR) was used to detect the expression of RhoC mRNA in the PHCC and PCL tissue of 30 patients with PHCC. Results The opacity density (OD)of RhoC mRNA expression in PHCC tissues was significantly higher than that in PCL tissures(P
RESUMO
Objective To investigate the influence of γ-interferon (γ-IFN) on liver cancer cell line (Hep-G2). Methods Observing the expression of Fas and Bcl-2 by γ-IFN-pretreated Hep-G2 cells via immunohistochemical stain; subsequently treating these cells with adrimysin, and observing the cell death rate and apoptosis of these cell by MTT and electroscopy. Results (1) γ-IFN up-regulating the expression of Fas protein and down-regulating Bcl-2 protein (P<0.05), and the sensitivity of pretreated hep-G2 cell to adrimysin was increased. Conclusions γ-IFN can rise the sensitivity of Hep-G2 to adrimysin via regulating the expression of Fas and Bcl-2.
RESUMO
Objective To establish an experimental model for exploring the role of RhoC gene in the invasiveness and metastasis of hepatocellular carcinoma.Method The RhoC gene was digested with restricted enzyme Hind III and XbaI,and direct cloned to pcDNA3.1.The recombinant vector (pcDNA3.1 RhoC) and the vector alone (pcDNA3.1) were transfected into HEPG2 cells with LIPFECTAMINETMReagent.After selected with hygromycin,resistant cloneies was obtained.The transcription and translation of RhoC gene were analysed with the reverse transcription PCR and immunohistochemical stain.Results The recombinant vectort (pc DNA3.1 Rhoc) express steadily in HerpG2 cells.Conclusions The modified tumor cells(HEPG2 RhoC) could be used to study the effect of RhoC protein on the invasiveness and metastasis of hepatocellular carcinoma.
RESUMO
Objective:To investigate the mechanism of Salvia Miltiorrhizae(SM) on protecting the renal injury of rats with obstructive jaundice.Methods:The 36 rats were randomly divided into control group,obstructive jaundice group and SM treated group.The renal injury of rats model with obstructive jaundice was established by ligating common bile duct.In the SM treated group,SM (2.0g/ d) was given through abdominal cavity.Animals were killed at the 7th,14th,21st and 28th day after operation respectively.The malondialdehyde (MDA) and superoxide dismutase (SOD) activity content of serum and renal tissue were determined,and the changes of renal function and histopathology were observed.Results:Comparing with non treated group,the serum and renal tissue SOD activity were markedly increased,and the MDA were obviously decreased(P
RESUMO
Objective:To evaluate the efficacy of dual intervention for the treatment of acute pulmonary embolism(PE) in patients with deep venous thrombosis(DVT) of lower limbs.Methods:Twenty five patients of DVT with PE were treated with combination of transcatheter thrombolysis and inferior vena cava filter placement.Results:All the filter procedures and catheter were successfully performed,without any complication.The clinical symptoms were markedly improved in all patients.Twenty three cases were followed up for 3-36 months after dual intervention,and no complications of interventional treatment or recurrent PE was found.Conclusion:The inferior vena cava filter placement is an effect method in preventing PE.Dual interventional procedures is a superior and effective method for managing the patients with PE.
RESUMO
Objectives:To investigate the therapeutic effect of somatostatin and parenteral nutrition on early postoperative inflammatory intestinal obstruction. Methods:16 patients(test group) were treated with somatostatin and parenteral nutrition.Somatostatin was administered in 6 mg doses daily through continuous intravenous drip and the formula of parenteral nutrition was provided according to clinical routines.The therapeutic effect was compared with that of previous 13 cases(control group) without use of somatostatin and parenteral nutrition. Results:The rates of transferring to operation in the test group and the control group were 12.5 % and 61.5%( P
RESUMO
Objectives:To investigate the effect of GH added early postoperative enteral nutrition on protein metabolism and immune function. Methods:Thirty six patients undergoing total or subtotal gatrectomy were randomly divided into three groups:traditional entertal nutrition (TEN),early postoperative enteral nutrition(EPEN),and GH added early postoperative enteral nutrition(GH+EPEN).Nutritional support lasted for 7 days.GH daily dose was 8 IU.Nutritional markers and immune markers were detected in several time points. Results:On postoperative day 4,the levels of Alb,Tf,TCL,and CD4/CD8 were all significantly increased in GH+EPEN as compared with those of TEN(all P
RESUMO
Objectives:To observe the influence of glutamine supplemented TPN on surgical patients. Methods:Thirty cases were randomly divided into two groups.One was glutamine supplemented TPN group(Gln TPN) and another group was standard TPN group (S TPN). Results:①Total lymphocyte count(TLC) was raised significantly in Gln TPN than in S TPN.②There were no differences in hepatic function,Hb and ALB between two groups.③There was no difference in recorery of intestinal function between two groups.④The patients recovered more rapidly from POF in Gln TPN group than in S TPN group. Conclusions:Glutamine supplemented TPN could increase TLC of surgical patients and remit POF more rapidly.
RESUMO
Objectives:To investigate the effect of growth hormone(GH) and parenteral nutrition (PN) on protein metabolism in patients with cirrhosis of liver. Methods:The test group received GH and PN,and the control group received PN only.All the treatments were maintained for 7 days.The serum levels of Alb,TFN and PA were detected on the day before the treatment and the day 3,5 and 7 after the treatment. Results:As compared with the day before the treatment,the levels of Alb,TFN and PA on the day 3,5 and 7 in the two groups were all increased.However,the changes in the test group were greater than those in the control group.As compared with the control group,the level of Alb on the day 7 and the levels of TFN and PA on the day 5 and 7 in the test group were significantly higher. Conclusions:The therapeutic value of combined use of GH and PN is much better than that of single PN in patients with cirrhosis of liver.
RESUMO
Objective To investigate the influence of ?-interferon (?-IFN) on liver cancer cell line (Hep-G2). Methods Observing the expression of Fas and Bcl-2 by ?-IFN-pretreated Hep-G2 cells via immunohistochemical stain; subsequently treating these cells with adrimysin, and observing the cell death rate and apoptosis of these cell by MTT and electroscopy. Results (1) ?-IFN up-regulating the expression of Fas protein and down-regulating Bcl-2 protein (P