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Objective To evaluate the effect of α7 nicotinic acetylcholine receptor (α7nAChR)agonists on lung injury caused by cardiopulmonary bypass (CPB) in rats.Methods Eighteen healthy clean-grade adult male Sprague-Dawley rats,weighing 350-400 g,were divided into 3 groups (n =6 each)using a random number table method:sham operation group (group S),CPB group and α7nAChR agonist PHA568487 group (group PHA).The rats underwent no CPB and were mechanically ventilated for 60 min in group S.PHA568487 0.8 mg/kg (diluted to 2 ml in normal saline) was intraperitoneally injected at 30 min before CPB,and then CPB was performed for 60 min in group PHA.Normal saline 2 ml was intraperitoneally injected at 30 min before CPB,and then CPB was performed for 60 min in group CPB.Blood samples were collected from the internal jugular vein for determination of serum interleukin-6 (IL-6) and tumor necrosis factor-alpha (TNF-α) concentrations by enzyme-linked immunosorbent assay.Lung tissues were obtained for microscopic examination of the pathologic changes and for determination of wet/dry weight ratio (W/D ratio) and matrix metalloproteinase-9 (MMP-9) expression (by Western blot).Results Compared with group S,the W/D ratio and serum concentrations of TNF-α and IL-6 were significantly increased,and the expression of MMP-9 was up-regulated in CPB and PHA groups (P＜0.05).Compared with group CPB,the W/D ratio and serum concentrations of TNF-α and IL-6 were significantly decreased,the expression of MMP-9 was down-regulated (P＜0.05),and the pathological changes of lung tissues were significantly attenuated in group PHA (P＜0.05).Conclusion α7nAChR agonists can reduce the acute lung injury caused by CPB in rats,and the mechanism may be related to down-regulating MMP-9 expression and inhibiting systemic inflammatory responses.
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Objective To compare the clinical intravenous anesthesia effect of sufentanil and remifentanil respectively, in order to provide data support for future clinical practice. Methods 91 surgery patients from February 2013 to October 2013 were selected and randomly divided into 2 groups, the one is sufentanil group (group A), and the other is remifentanil group(group B). The change of hemodynamic indexes, catecholamine concentration and woke up time after surgery in two groups were monitored and compared. Results Compared with T 0, levels of SP, DP, HR in two groups were signiifcantly lower (P<0.05), at the moment of T 2, the levels of SP, DP, HR level got signiifcantly higher(P<0.05), even surpass T 0(P<0.05). And the range of the rise in group A was signiifcant than that in group B(P<0.05).Epinephrine and norepinephrine of group A in T 2 and T 6, were higher than T 0 (P<0.05). The spontaneous breathing recovery time was, (5.2±1.9)min, open eyes’time, (7.2±2.0) min, and conscious time was(14.6±2.3)min in group B, which were lower than that of group A(t=14.382, 14.934, 14.382, P<0.01).There were no adverse reactions in both groups. Conclusion Remifentanil has good anesthetic effect, postoperative patients wake up soon, self breathing early. The change of hemodynamic index and catecholamine index are stable and beneifcial to internal environment balance.
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Objective To investigate the resistance rate of Helicobacter pylori (Hp) to clarithromycin and its correlation with point mutations in 23S rRNA gene. Methods Hp was cultured from gastric biopsy specimen obtained from 189 patients undergoing upper gastrointestinal endoscopy. The DNA of 11 clarithromycin sensitive Hp and 19 clarithromycin resistant Hp was extracted, and 23S rRNA was amplified and sequenced. Results The rate of clarithromycin resistance in cultured Hp was 29. 2%. Point mutations in 23S rRNA gene were found in 17 clarithromycin resistant Hp strains. The proportion of A to G mutation was 36.8%, G to A of 21.5%, C to T of 15.8%, A to C of 10.5% and T to C of 5.3%. No point mutation in 23S rRNA was detected in other 2 clarithromycin resistant and 11 sensitive Hp strains. Conclusion The resistance to clarithromycin is common in Hp, and point mutations in 23S rRNA gene of Hp are frequent in clarithromycin resistant strains, with most prevalent mutations of A to G and next G to A.