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Objective To study the characteristics of hemogram and myelogram with Brucellosis patients.Methods 25 Brucellosis patients' bone marrow and peripheral blood smears were observed,at the same time a deep clinical inverstigation on the patients was conducted. Results The counts of blood cells were decreased in part pa-tients, and a typical lymphocytes were found in peripheral blood of 64% patients. The marrow proliferative activities were normal(92% ) or higher(8% ),and all patients had poisonous granules. Red cell proliferative activities were normal(68% ) or lower(32% ). The counts of megakaryocytes were normal,but the maturity of megakaryocyte was hindered. All patients' histocytes were increased at different degrees. NAP were increased. Conclusion The changes of hemogram and myelogram have clinical significance for diagnosis of Brucellosis, but primary hematologic disease with Brucellosis should be distinguished.
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ObjectTo study the adsorptive properties of spherical activated carbon on some effective ingredients from Chinese materia medica (CMM) and to approach the application feasibility to CMM purification. Methods The spherical activated carbon with high strength has been successfully prepared by the carbonation and activation of polymeric resin. The apparent and theoretical adsorption capacities under the condition of static operation were investigated by Langmuir monolayer adsorption model. Elution by 75% alcohol was studied. Results The static adsorption capacities of the berberine hydrochloride and dioscin were 35.46 mg/g and 47.12 mg/g, and theoretical adsorption capacities were 96.16 mg/g and 102.04 mg/g, respectively. The apparent adsorption amount of rutin was 40.88% mg/g. The elution ratio were 83.71%, 91.45%, and 87.69%, respectively. Conclusion The spherical activated carbon adsorbent shows better comprehensive adsorption properties and can be used to purification.
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Objective To apply the membrane technology for purifying rough cordyceps polysaccharides solution,the optimal operation condition and effective cleaning method were researched.MethodsTo mainly analyze the flux of membrane and its recovery.Results The optimal experiment operation condition obtained is 60℃,pH value 7-8,and pressure 0.3-0.4 MPa,by which the polysaccharides recovery was about 30%,more than by traditional ethanol extraction,and the most impurities as infusibility substance and protein colloid etc.were taken out.Moreover,the flux of membrane recovery can be above 90% after 80℃ water and 1% NaOH solution was alternately used to wash the fouling membrane under intermittent little back-pulsing. Conclusion The process of membrane technology purifying rough cordyceps polysaccharides is simpl and feasible.
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AIM:To characterize the inclusion complex of volatile oil of Rhodiola crenulata/?-cyclodextrin. METHODS: Some analytical methods,such as DTA,IR,GC,TLC were apllied to the investigation before and after the inclusion. RESULTS: The difference between the inclusion and physical mixture in differential thermal(analysis,) infrared spectra,gas chromatogram and thin layer chromatogram. CONCLUSION: The inclusion of Rhodiola crenulata volatile oil has the characteristic of intramolecular inclusion.
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AIM: To optimize the extraction process for coumarins in Angelica Dahurica by supercritical CO 2 as guided by the contents of oxyimperatorin, imperatorin and the total coumarins in the extract. METHODS: HPLC was applied to determine the contents of oxyimperatorin and imperatorin. orthogonal design was applied to optimize SFE process. Range and variance analysis as well as stepwise nonlinear regression analysis were applied to deal with experimental results. RESULTS: The optimum process was established as following: 21MPa as extracting pressure, 50℃ as extracting temperature, 3.5h as extracting time, 20 mesh as pulverized degree and 6.5MPa as separating pressure. The relative error between experimental data and calculated value was less than 5%. CONCLUSION: Changes in extracting pressure, temperature, time, pulverized degree would affect the extracting results remarkably. Some factors had interactions on the extraction of coumarins in Angelica Dahurica . Regression equation was reasonable and could forecast results precisely.
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Objective: To establish the relationship equation of yield and extraction time of plant volatile oil. Methods: By means of the rate eqation of substance transfer, the relationship equation of yleld (v) and extraction time (t) of volatile oil was accomplished: ln(V s-V)=-K t+A,K and A were parameters. Results: The experiment of volatile oil of Rhizoma Nardostachyos indicated that A was 0.44, K was 2.7?10 -3 , and the correlation coefficient (r) was -0.99 whereas A was 1.33, K was 3.62?10 -3 and r was -0.99 in the experiment of volatile oil of Flos Syzygii Aromatici. Conclusion: This relationship equation is able to reflect the relationship between the yield and extraction time of volatile oil.