RESUMO
OBJECTIVE:To establish a method for the simultaneous determination of paeoniflorin and paeonol in Dirda pill, and provide reference for improving its quality control standard. METHODS:HPLC was performed on the column of ZORBAX C18 with mobile phase of acetonitrile-0.1% phosphoric acid (gradient elutio) at a flow rate of 0.8 ml/min,detection wavelength was 230 nm(paeoniflorin),274 nm(paeonol),columne temperature was 35 ℃,injection volume was 20 μl. RESULTS:The linear range was 0.514 8-1.544 5μg for paeoniflorin(r=0.999 2)and 0.643 2-1.960 4μg for paeonol(r=0.999 8);the limits of quantifi-cation were 0.135 6,0.126 4 μg,limits of detection were 0.067 8,0.063 2 μg;RSDs of precision,stability and reproducibility tests were lower than 2%;recoveries were 95.78%-97.27%(RSD=0.62%,n=6) and 97.38%-98.38%(RSD=0.42%,n=6). CONCLUSIONS:The method is simple with good reprosucibility and high sensibility,and can be used for the simultaneous deter-mination of paeoniflorin and paeonol in Dirda pill.
RESUMO
AIM:To establish the quality standard for Guanxin Plaster(Radix et Rhizoma Salviae miltiorrhizae,Rhizoma Chuanxiong,Olibanum,etc). METHODS: Radix et Rhizoma Salviae miltiorrhizae;Rhizoma Chuanxiong and Radix Angeliae pubescentis of Guanxin Plaster were identified by TLC.Camphor,menthol and borneolum syntheticum were cletermined by GC together. RESULTS: The characteristic identification by TLC was distinct and highly specific.Camphor showed a good liner relationship at a range of 0.095 ?g-0.95 ?g,(r=0.999 7,n=5).Menthol showed a good linear relationship at a range of 0.123 ?g-1.23 ?g,r=0.999 6,n=5).Borneolum syntheticum showed a good liner relationship at a range of 0.202 ?g-2.02 ?g,(r=0.999 7,n=5).The average recoveries were 99.6%(RSD=1.9%) for camphor,98.4%(RSD=2.0%) for menthol,98.2%(RSD=3.8%) for bomeolum syntheticum. CONCLUSION: The method is reliable,accurate and specific.It can be used for quality control of Guanxin Plaster.