Comparative study on Development of Mouse Embryos in Conventional Medium versus Vero Cell Coculture / 대한산부인과학회잡지

OBJECTIVE: To evaluate mouse embryos development in conventional medium IVF-20 versus vero cell coculture. METHODS: Female ICR mice aged 6 to 8 weeks, were stimulated with 5IU PMSG and 48 hours later were injected 5IU of hCG, then female and male mice were mated. At 48 hour post-hCG injection, oviducts were dissected out and 2-cell embryos were flushed. The 2-cell embryos were cultured in IVF-20 media or media containing vero cell (African green monkey kidney epithelial cell lines) for 120 hours. Coculture techniques have been applied in mouse 2-cell embryos culture used vero cell lines. RESULTS: 1. After 48 hours culture, 60.7% and 55.7% of 2 cell embryos developed to 4 cell and morulae stage, respectively, in IVF-20 culture medium, but significantly less embryos developed to 4 cell (47.6%, p<0.05) and momlae (42.9%, p<0.05) in vero cell coculture. 2. After 72 hours culture, 51.6% of 2 cell embryos developed to blastocyst and expanded blastocyst in IVF-20 culture medium, but significantly less embryos developed to blastocyst and expanded blastocyst (25.9%, p<0.01) in vero cell coculture. 3. After 96 hours culture, 37.7% and 32.6% of 2 cell embryos similar developed to expanded blastocyst and hatching in IVF-20 culture medium and vero cell coculture, respectively. 4. After 120 hours culture, 36.9% and 37.4% of 2 cell embryos similar developed to expanded blastocyst and hatching in IVF-20 culture medium and vero cell coculture, respectively. CONCLUSION: There was no difference of embryo development rates between the two culture groups. IVF-20 medium alone gives a benefit to the viability of an embryo compared with a vero cell coculture.

A Study on the Translocation of Human Chromosome / 대한주산의학회잡지

OBJECTIVE: The aim of this study was to evaluate the relation between the chromosomal translocation and the outcome of pregnancy. METHODS: Between 1989 and 2001, 26 translocation carriers have been detected in our center and Jin Obstetrics and Gynecology Clinic. The subjects consisted of 26 couples that included 14 reciprocal translocation carriers and 12 Robertsonian translocation carriers. The balanced translocation carriers were analyzed retrospectively. RESULTS: The most frequent indication for parental chromosomal examination was repeated abortions or fetal death(57.7%). In contrast to couples with reciprocal translocations, a high excess of female over male carriers was found in the group of Robertsonian translocations. The rates of miscarriages (68.0%) in prior pregnancies were significantly higher than the birth rates of morphologically normal newborns(16.0%). Prenatal chromosomal examination in subsequent pregnancies revealed that 24.0% of the fetuses showed normal karyotypes, 56.0% of the fetuses showed balanced translocations and 20.0% of the fetuses showed a chromosomal imbalance. The unbalanced karyotypes consisted of three trisomies, one partial trisomy and one duplication. There was no case of partial monosomy in this study. CONCLUSIONS: The risk of unbalanced chromosome abnormalities was greater in our study than the empirically known risk. Prenatal examination is always indicated in carriers of any type of translocation.

Vaginal Removal of the Pedunculated Submucous Myoma: Twisting-off Method / 대한산부인과학회잡지

OBJECTIVE: To evaluate the efficacy of the twisting-off method in transvaginal removal of the pedunculated submucous myoma. METHODS: After paracervical block and vaginal dressing with Povidone iodine, the myoma was grasped at its widest diameter and twisted to avulse it from its attatchment. Several kinds of grasping instruments, ie. kelley clamp, tenaculum, ring forcep, was used. If there was bleeding from its detachment site, vaginal gauze or tampon was inserted for bleeding control. RESULTS: The procedure was perfomed successfully on all 33 patients (100%). In most patients, bleeding was scanty in amount. But in one patient, emergency hysterectomy was performed because of massive uterine bleeding. The myoma varied greatly in size. Largest volume was 62.24 cm3, and mean volume was 13.79 cm3. Pathology revealed classic leiomyomas in most cases. Some showed endocervical polyp or endometrial polyp. Follow up examination was done, and its mean interval was eighteen weeks and no patient was recurred. CONCLUSION: The transvaginal removal of pedunculated submucous myoma utilizing grasping instruments may be an effective and safe procedure and the complication is minimal. Twisting-off method was may be a safe alternative to abdominal myomectomy and hysterectomy. Reproductive capacity can be preserved, also.

A case of the surgical treatment for deep vein thrombosis in the leg caused by huge uterine adenomyosis / 대한산부인과학회잡지

Uterine adenomyosis is a common benign pelvic tumor in women. One of the complications that may be infrequently associated with the huge pelvic mass is venous stasis of the lower extremities and may develop thrombophlebitis secondary to pelvic compression. Intravascular thrombosis in the deep vein of the legs is a serious illness that sometimes cause death due to acute pulmonary thromboembolism. Deep vein thrombosis (DVT) has been reported to be closely related to pregnancy, surgical procedure, long term bedrest, obesity, and oral contraceptives. However, there is few report about deep vein thrombosis caused by huge uterine adenomyosisWe report a case of deep vein thrombosis caused by huge adenomyosis was treated by hysterectomy, thrombectomy, and thrombolysis with a brief review of the literature.