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Chinese Journal of Biotechnology ; (12): 1048-1058, 2012.
Artigo em Chinês | WPRIM | ID: wpr-342417

RESUMO

We used ribosome engineering technology, with which antibiotic-resistant strains are resulted from mutations on microbial ribosome, to screen a high butanol-producing Clostridium strain. A novel mutant strain S3 with high butanol production and tolerance was obtained from the original Clostridium acetobutylicum L7 with the presence of mutagen of streptomycin. Butanol of 12.48 g/L and ethanol of 1.70 g/L were achieved in S3, 11.2% and 50%, respectively higher than the parent strain. The conversion rate of glucose to butanol increased from 0.19 to 0.22, and fermentation time was 9 h shorter. This caused an increase in butanol productivity by 30.5%, reaching 0.24 g/(Lh). The mutant butanol tolerance was increased from 12 g/L to 14 g/L, the viscosity of fermentation broth was dramatically decreased to 4 mPa/s, 60% lower than the parent strain. In addition, the genetic stability of mutant strain S3 was also favorable. These results demonstrate that ribosome engineering technology may be a promising process for developing high butanol-producing strains.


Assuntos
Butanóis , Metabolismo , Clostridium acetobutylicum , Genética , Metabolismo , Fermentação , Engenharia Genética , Mutação , Proteínas Recombinantes , Genética , Ribossomos , Genética , Estreptomicina , Farmacologia
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