RESUMO
Malaria vaccines are being developed against different stages in the parasite's life cycle. Although not directly protective ,the sexual stage vaccines would induce antibodies that would prevent infection of mosquitoes when ingestedin a bloodmeal containing sexual stage parasites. pfs25 has been tested to be an important candidate antigen for malarial transmission blocking vaccine . In this report we analyzed the complete code of pfs25 gene of Plasmodium falciparum PFD-3/YN isolate. The result shows that the gene encoding pfs25 of PED-3/YN isolate has a mutant which generates a PstI endonuclease restriction site and shares 99.2％ nucleotide homology with that of NF4 isolate.
RESUMO
We measured the Na-K-ATPase activity in erythrocyte membrane from 21 diabetic patients and 21 normal subjects with molybdenum blue colorimetry for inorganic phosphorus and Lowry's method for protein. Changes of this enzyme in erythrocyte membrane before and after treatment were also observed ia 13 diabetics. Our results demonstrated that the Na-K-ATPase activities in erythrocyte membrane from diabetic patients were lower than that from normal controls and no significant difference in Na-K-ATPase activity was observed before and after treatment in diabetics. Experiments in vitro revealed that lowered Na-K-ATPase activity in erythrocyte membrane from diabetics was not related to the serum insulin and glucose levels.