Adaptation of ovarian cancer cell line SKOV-3ipl cells to hypoxia and its correlation with VEGF and Bcl-2 protein expressions / 中国癌症杂志

Purpose:To investigate the adaptation of ovarian cancer cell line SKOV 3ipl cells to hypoxia and its correlation with the expressions of VEGF and Bcl 2 protein.Methods:The monolayer SKOV 3ipl cells plated in culture bottles were placed in an anaerobic system consisting of 85%N 2, 5%CO 2, 10%H 2 with very low oxygen concentration (

Hypoxia-induced apoptosis and anti-apoptosis in ovarian cancer cell line SKOV-3ipl cells and its correlation with cell cycle / 中国癌症杂志

Purpose:To investigate hypoxia induced apoptosis and anti apoptosis in ovarian cancer cell line SKOV 3ipl cells and its correlation with cell cycle changes.Methods:The monolayer SKOV 3ipl cells plated in culture bottles were placed in an Anaerobic system insulated with 85% N 2,5% CO 2,10% H 2 and very low O 2 concentration (100 ppm). Apoptosis and cell cycles were measured with FITC Annexin V/PI double stain and DNA prep stain Flow cytometry in cells cultured in anaerobic condition. Results:Apoptosis in cells cultured in hypoxia condition for 0h,8h,16h,24h. were (0.32?0.01)%,(8.29?0.87)%,(4.23?0.75)%,(0.59?0.08)% respectively. Between 8h or 16h and 0h, P