3D Histology Using the Synchrotron Radiation Propagation Phase Contrast Cryo-microCT / 체질인류학회지

3D histology is a imaging system for the 3D structural information of cells or tissues. The synchrotron radiation propagation phase contrast micro-CT has been used in 3D imaging methods. However, the simple phase contrast micro-CT did not give sufficient micro-structural information when the specimen contains soft elements, as is the case with many biomedical tissue samples. The purpose of this study is to develop a new technique to enhance the phase contrast effect for soft tissue imaging. Experiments were performed at the imaging beam lines of Pohang Accelerator Laboratory (PAL). The biomedical tissue samples under frozen state was mounted on a computer-controlled precision stage and rotated in 0.18° increments through 180°. An X-ray shadow of a specimen was converted into a visual image on the surface of a CdWO4 scintillator that was magnified using a microscopic objective lens (X5 or X20) before being captured with a digital CCD camera. 3-dimensional volume images of the specimen were obtained by applying a filtered back-projection algorithm to the projection images using a software package OCTOPUS. Surface reconstruction and volume segmentation and rendering were performed were performed using Amira software. In this study, We found that synchrotron phase contrast imaging of frozen tissue samples has higher contrast power for soft tissue than that of non-frozen samples. In conclusion, synchrotron radiation propagation phase contrast cryo-microCT imaging offers a promising tool for non-destructive high resolution 3D histology.

Anthropometric Characteristics of the Maxillary Sinus in Korean / 체질인류학회지

The maxillary sinus is an anthropological landmark because of difference of volume of maxillary sinus among persons of different ethnic origin. This study was undertaken to provide morphological characteristics of Korean's maxillary sinus. The size and volume of maxillary sinus were measured in 44 Korean skulls 23 males and 21 females from cadaver. The shapes of the maxillary sinus were classified according to Anagnostopoulou et al. (1991) and the sizes of the medial antral wall area of the maxillary sinus were classified according to Fernandes (2004b). The mean volume of maxillary sinus was 12.83+/-5.41 cm3, which was significantly different between males and females. Bilateral symmetry of the volume of the maxillary sinus was observed in the 70% of the specimens. The width of maxillary sinus was 26.59+/-4.96 mm. The height of maxillary sinus was 26.65+/-6.16 mm. The anteroposterior length was 32.84+/-4.96 mm. The anteroposterior length measured on C-Arm radiography was 36.99+/-4.72 mm. The shape of maxillary sinus was semi-ellipsoid and the majority (50%) of the shape of medial antral wall area of maxillary sinus was the type of small. The results of this study could be useful to clarify the anthropological characteristics of the maxillary sinus in Korean, and to provide an index of ethnic classification for the medial antral wall area.

Localization of Laminin and Laminin beta 1 chain on Gomeruli of Developmental Rat Kidney / 체질인류학회지

Laminin, an extracellular matrix glycoprotein composed of three polypeptide chains such as alpha , beta, and gamma is distributed in basement membranes of epithelium, muscle, and nervous tissues. Laminin functions as an extracellular cytoskeleton and regulates the differentiation and polarization of cells adjacent to the basement membrane. Along with type IV collagen and heparan sulfate proteoglycan, laminin forms a spike -like structure in the renal glomerular basement membrane (GBM). It has been previously demonstrated that the distribution and immune reaction of laminin are changed in response to the conditions of glomerulonephritis and that laminin plays a role in the reformation of GBM as well as the regeneration of renal glomerular cells. In the present study, the profile of expression and distribution of laminin/laminin beta1 chain were examined in different developmental stages and upon adriamycin administration. Kidney obtained from fetuses (16, 18, and 20 days old) and infants (1 and 7 days old) of Sprague -Dawley rats were either cryosectioned for immunohistochemical assays or ultrathin -sectioned for electron microscopy using immunogold staining methods. The results were as follows: 1. Intensive expression of laminin was observed in the GBM and surrounding mesenchymal tissues obtained from 16, 18, and 20 days old fetuses and in the glomerulus from one day neonates, whereas the level of staining decreased in the glomerulus from 7 days old infants. 2. Immunogold particles were observed in the comma -shaped nephron, in particular in cisternae of rough endoplasmic reticulum, vesicles and nuclear membrane of endothelial cells and mesangial cells obtained from 18 days old fetuses. 3. The immune reactions of laminin beta1 chain were trace detected in the kidney from fetuses (16, 18, and 20 days old) and weakly in tissues surrounding blood capillary and mesangial tissues from one day old neonates. 4. After 24 hours following adriamycin treatment, the reactivity of laminin was slightly enhanced in the renal glomerulus, when compared with that of untreated controls. This enhancement persisted up to 1 week of adriamycin treatment. Laminin beta1 chain was weakly detectable, while further treatment with adriamycin for another 24 hours reduced the intensity of laminin beta1 chain. Taken together, these results suggest that laminin is localized in the GBM at the high level during early fetal stages but the expression levels decrease after birth. Moreover, administration with adriamycin may result in an increase in the immune reactivities of laminin and laminin beta1 chain by renal tissue damage followed by renal regeneration.