RESUMO
Background: Mycobacterium avium subsp paratuberculosis [M.a.p] causes chronic intestinal inflammation in dairy cattle and eventually leads to Johne's disease. In some studies, mentioned bacteria has been detected and isolated from milk. As regards, during the milking process; milk could get contaminated with chattels feces, therefore the extent of contamination of milk depends on hygienic methods prior and during of milking and the preparation and delivery to the consumer market processes too. So, the aim of this study was determined the prevalence of mentioned bacteria in pasteurized milk in Ardebil province by PCR method for the first time
Methods: In total; 330 commercially pasteurized milk samples were purchased from various production centers of Ardebil province and 50ml from each sample were centrifuged for M.a.p DNA extraction. Extracted DNA was evaluated for the presence of M.a.p's specific sequence of IS900 by two protocol of PCR assay
Results: M.a.p DNA was detected in 33cases [10% of samples] by assay with F90,F91 primers, 22 cases [6.66% of samples] by assay with FP25, RP26 primers and 11 cases [3.33% of samples] by assay with both pairs primers
Conclusions: The results obtained from this study; demonstrate a relatively high occurrence of M.a.p in pasteurized milk. In addition, regarding the possible etiological role of M.a.p in the development of Crohn's diseases [Infectious Bowel Disease], it is considered as a serious concern for public health
RESUMO
Background and Objectives: After Staphylococcus aureus and E.coli, Pseudomonas aeroginosa has been the third most common cause of nosocomial infections and their active opportunistic transport, occurs due to the disregarding public health, So this study was done in order to investigate the antibacterial effects of Essential oils and Ethanol extracts of the Native Plants, Ziziphora Clinopodioides on the isolated bacteria from the urinary tract of laboratory rats, as an experimental model
Materials and Methods: Under sterile conditions, required amount of urine collection was taken from 40 healthy Wistar rats and after identifying the isolates with standard microbiologic methods, to determine the antimicrobial effects of essential oils and ethanol extracts of that native plants on the isolates, MIC, MBC and antibiogram tests was performed
Results: The results showed that both essential oils and ethanol extracts of Ziziphora Clinopodioides have inhibitory and bactericidal effects on all isolated bacteria except for Pseudomonas aeruginosa and comparison of the inhibitory and bactericidal effects of these compounds revealed that the essential oils of this plant compared to its extracts is able to inhibit the growth isolated bacteria with low concentrations. Also, there was a significant difference between the antibacterial activity of these plants [P<0.05]
Conclusions: It seems that we can use the compounds of Ziziphora Clinopodioides as the appropriate antibacterial materials against bacteria such as opportunistic pathogens [except for Pseudomonas aeruginosa], that may cause the human infections