RESUMO
Purpose@#: This study aims to provide basic data for effective nursing interventions and improvement of nurses’ work by exploring their work experiences in single-room-structured intensive care units (ICU) through focus group interviews. @*Methods@#: Data were collected through two focus group discussions conducted from March to November 2020 with 13 ICU nurses. Interviews were audio-recorded and analyzed thematically by investigators. @*Results@#: Through content analysis, 15 sub-categories and 6 categories were formed. Two themes, “positive experiences patients care in an independent space” and “difficulties in nursing work according to space separation of patients” emerged. There are positive aspects of single-room-structured ICUs, but it was found that practicing nurses had difficulties and required specialized nursing competencies. Therefore, efforts to reduce the burden of nurses in single-room-structured ICUs are necessary. @*Conclusion@#: The limitation of this study is that it was conducted in a single hospital because single-room-structured intensive care units are uncommon in Korea. However, this study is of great significance as a basis for establishing guidelines on the efforts required from nurses, hospitals, and governments single-room-structured ICUs in the future.
RESUMO
Normal human B cells produce autocrine growth factor in response to Staphylococcus aureus Cowan I strain (SAC). However, the functional role and molecular nature of the B cell derived-B cell growth factor (B-BCGF) are largely unknown. We have tried to investigate the nature of B-BCGF using mAb for several years. We report here that B- BCGF is capable of binding to hemoglobin (Hb). The concentrated culture supernatant from tonsillar B cells stimulated with SAC for 24 h was loaded into the fast protein liquid chromatography and ion-exchange chromatography. The peak with BCGF activity was shown to have a M.W. of 16-18 Kda in polyacrylamide gel electrophoresis followed by silver stain. Amino acid sequence of the fraction was found to identical to human hemoglobin (Hb) by more than 85%. However, Hb itself had no BCGF activity. The presence of Hb in culture supernatant was due to the contamination of SRBC during B cell purification. SRSC were completely removed from B cells by percoll-gradient centrifugation and B cells were stimulated with SAC and exogenous Hb was added to the cultures. The Hb fraction from FPLC again showed a BCGF activity. These data strongly suggested that BCGF binds to Hb. We confirmed this in dot blot as well as Western blot. The M.W of Hb-binding BCGF was 20 Kda. This information may provide a rapid progress in research of BCGF.