4-Hydroxybenzaldehyde, One of Constituents from Gastrodiae Rhizoma Augments Pentobarbital-induced Sleeping Behaviors and Non-rapid Eye Movement (NREM) Sleep in Rodents

In the previous experiments, we reported that ethanol extract of Gastrodiae Rhizoma, the dried tuber of Gastrodia ElataBlume (Orchidaceae) increased pentobarbital-induced sleeping behaviors. These experiments were undertaken to know whether 4-hydroxybenzaldehyde (4-HBD), is one of the major compounds of Gastrodiae Rhizoma increases pentobarbital-induced sleeping behaviors and changes sleep architectures via activating GABA(A)-ergic systems in rodents. 4-HBD decreased locomotor activity in mice. 4-HBD increased total sleep time, and decreased of sleep onset by pentobarbital (28 mg/kg and 40 mg/kg). 4-HBD showed synergistic effects with muscimol (a GABA(A) receptor agonist), shortening sleep onset and enhancing sleep time on pentobarbital-induced sleeping behaviors. On the other hand, 4-HBD (200 mg/kg, p.o.) itself significantly inhibited the counts of sleep-wake cycles, and prolonged total sleep time and non-rapid eye movement (NREM) in rats. Moreover, 4-HBD increased intracellular Cl- levels in the primary cultured cerebellar cells. The protein levels of glutamic acid decarboxylase (GAD) and GABA(A) receptors subunits were over-expressed by 4-HBD. Consequently, these results demonstrate that 4-HBD increased NREM sleep as well as sleeping behaviors via the activation of GABA(A)-ergic systems in rodents.

Bacteriological Mass Survey on Prostitutes for American Soldiers / 예방의학회지

This survey was carried out on about 240 prostitutes for American soldiers in Bong-Deok Venereal Diseases Clinic, Daegu Korea, through 2 times for two months from October and November 1974. The culture method was used identifing Neisseria and Mimeae species carbohidrate fermentation test was ferformed with phenol red broth base added human serum. Resistance test was informed with disc method in chocholete agar plate. The discs were made by Jong-Geon Dang Pham. Co.. Seoul, Korea. The carrierate of Neisseria genorrheae was 9.8% and there were relatively many carriers of Mimeae. If the direct smear is used only in microscopic diagnosis, it is easily confused to differentiate Gonococcus and Mima, a kind of coccobacilli. Gonococcus resistance to penicillin G was found in one out of 5 cases tested. The most of Mimeae was resist to penicillin G, but the broad spectrum antibiotics such as chloramphenico1 and gentamycin etc was moderatly sensitive.

A Study of Venereal Diseases Control for Prostitutes and Playmates of Foreign Soldier in a Urban Area / 예방의학회지

No abstract available.