Evaluation of an Automated Instrument, PREVI Isola(R) for Inoculation of Body Fluids and Urine Samples onto Agar Plates

BACKGROUND: In most clinical microbiology laboratories, inoculation of specimens on plates is performed manually and is a time-consuming process. The efficiency of this process can be improved by using an automated instrument. Currently, several automated instruments have been introduced for inoculation of samples. In this study, we have evaluated an automated instrument, PREVI Isola(R) (Biomerieux, France), used for inoculation of body fluids and urine specimens. METHODS: Both manual and automated instrument methods were used to inoculate 74 body fluid and 204 urine samples. Precision was evaluated by testing 3 types of urine samples (A, 6x10(3) colony-forming units (CFU)/mL; B, 3x10(4) CFU/mL; and C, >10(6) CFU/mL) in replicates of 20. Results of the 2 methods were compared by counting the isolated colonies on agar plates after incubation. The time required for both methods was also compared. RESULTS: The coefficient of variation (CV) of samples A, B, and C examined using the automated instrument method was 176.1%, 18.1%, and 12.6%, respectively. The sensitivity and specificity of testing body fluid samples were 77% and 100%, respectively, and those of urine samples were 87% each. The time required for testing 15 body fluid specimens and that for inoculation of each specimen was 9.7 min shorter using PREVI Isola(R) than using the manual method. CONCLUSIONS: The results of body fluid and urine culture by inoculation using the automated instrument, PREVI Isola(R), showed relative good agreement with those obtained using the manual method. The use of PREVI Isola(R) would be expected to reduce the time and labor involved in inoculating various kinds of specimens.

Simultaneous Isolation of Vibrio vulnificus and Vibrio parahaemolyticus in Blood from a Liver Cirrhosis Patient: Importance of Detection and Identification of Both Species / 대한진단검사의학회지

A 56-year-old woman with underlying liver cirrhosis was hospitalized with chief complaints of fever, which developed after eating raw fish on the previous day. On physical examination, she showed hypotension. Vibrio vulnificus and Vibrio parahaemolyticus strains were simultaneously isolated from blood cultures, and the patient recovered after treatment with antibiotics including cefotaxime. To our knowledge, simultaneous isolation of both V. vulnificus and V. parahaemolyticus from the blood has never been documented before in Korea or any other countries. When blood cultures from a patient with underlying disease such as liver disease show growth of gram-negative bacilli in the summer months, microbiologists in Korea, where Vibrio infection is prevalent, should be aware of the possibility that V. vulnificus and other Vibrio spp. can be isolated simultaneously. An accurate identification of all isolates is important, because antimicrobial susceptibility patterns, severity and prognosis of the infection are different significantly depending on species.