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Journal of the Egyptian Society of Parasitology. 2002; 32 (1): 247-57
em Inglês | IMEMR | ID: emr-59721

RESUMO

In this study, ELISA was used for the detection of antibodies against the immunodominant surface antigen 1 [SAG 1, synonymous P30] of Toxoplasma gondii and peroxidase-conjugated protein G was used instead of commercially unavailable enzyme-conjugated anti-dromedary antibody. A latex agglutination test was employed to select 20 seronegative control animals and peroxidase-conjugated protein A was used for comparison with protein G. The overall seroprevalence rate was 31.4%, males had, to some extent, a higher seropositive rate than females. Seropositive camels sampled in winter had significantly higher antibody levels than those sampled in summer. ELISA values using protein G and protein A conjugates were significantly correlated. The results were discussed


Assuntos
Animais , Masculino , Feminino , Anticorpos Antiprotozoários , Antígenos de Superfície , Ensaio de Imunoadsorção Enzimática , Testes Sorológicos , Estações do Ano , Camelus , Toxoplasma/imunologia
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