RESUMO
Bone marrow cells and cultures of embryo skin and lung cells from DBA/2 mice were obtained for chromosome analysis in our studies. The specimens were banded by Giemsa staining following trysinization, which produced well-scattered and sharply banded mitotic figures ranging from early metaphase to mid-metaphase. Over 435 bands Within the mouse karyotype can be distinguished. Idiogram of G-banding patterns were constructed on the basis of large amount of karyotype analysis. The features of banding patterns of the individual chromosomes are presented and those chromosomes with similar banding patterns are contrasted to avoid possible misidentification.
RESUMO
The mitotic chromosomes of the ratlike hamster were studied under light microscope. The observations revealed that chromosome number of this species of genus cricetulus was 2n=28. The autosome pairs 1～11 were acrocentric and progressively decreasing in size, whereas the pairs 12 and 13 were four extremely small submetacentric chromosomes. The sex chromosome pairs were XY and XX for male and female animals respectively The X chromosome (Large subtelocentric) and Y chromosome (Submetacentric of medium size) were easily distinguishable from each other as well as from all autosomes. The poessible use of these outstanding chromosomal characteristics was briefly discussed.
RESUMO
Since rabbit (Oryctolagus cuniculus) possess many fine biological characteristics, they have been used in the studies of tumour, gene mapping and evolution. All of these need us to know the high-resolution G-banding pattern of rabbit chromosomes. Primary lung fibroblasts of new born rabbits were cultivated in RPMI 1640 medium supplemented with 20% new born bovine serum. In order to get the synchronized cells, chemical(MTX, TdR) method and physical (Cold Shock) method were used. By using trypsin-Giemsa banding-technique, we obtained fine high-resolution G-banding karyotypes of rabbit. The bands of rabbit chromosomes were analyzed and described. An idiogram which contains 583 bands of high-resolution G-banding chromosomes of rabbit has been made. The feature of rabbit choromosome bands and the method uesd to get the high resolution banding chromosomes were briefly discussed.