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Chinese Journal of Biotechnology ; (12): 758-760, 2002.
Artigo em Chinês | WPRIM | ID: wpr-256124

RESUMO

Two transgenic mouse strains, in which the expression of human factor IX (hFIX) in the milk were different significantly, were bred, and the foreign gene integration as well as the content of hFIX in the milk were detected by PCR, Southern blot, FISH and ELISA, respectively. The results showed that approximately 50% offsprings were transgenic positive. Foreign gene integrated in mouse chromosomes was intact. The hFIX expression of each mouse in the same strain was different, the content of hFIX in the milk was (43.32 +/- 5.41) microgram/mL in FIX-33 transgenic strain and (1.16 +/- 0.45) microgram/mL in FIX-124 transgenic strain. Meanwhile, the hFIX gene expression between the two strains was different remarkably (P < 0.01). We conclude that the characteristics of inheritance and expression in the founder were able to be transferred to their offsprings stably.


Assuntos
Animais , Feminino , Humanos , Camundongos , Fator IX , Genética , Hibridização in Situ Fluorescente , Camundongos Transgênicos , Leite , Química , Proteínas Recombinantes
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