Effects of Pb2+ stress on seed germination & seedling growth of Rabdosia rubescens / 中国中药杂志

The seeds of Rabdosia rubescens were as the materials to research the impacts of different lead (Pb2+) concentrations(0, 135, 270, 540, 1 080 mg x L(-1)) on seed germination and seedling growth. The results show that: Low concentration of lead had no obvious effect on early germination of the seed, the germination vigor and germination speed were lightly higher but not significantly differed at the level of Pb concentration 135 mg x L(-1) with control group; Mid-high concentration of Pb solution (270-1 080 mg x L(-1)) significantly inhibited the seed germination and seedling growth, which reduced the seed germination rate, germination vigor, germination index, embryo root length and shoot length, growth index with increasing of Pb concentrations. There was a inhibitory effect on embryo shoot length and root length at mid-high lead concentrations stress, and stronger inhibitory effect on root , which was more sensitive than shoot to Pb stress(P < 0.05). Pb bioaccumulation coefficient (BC) was 0.76-2.59, increased with concentration of Pb; Pb enrichment in seedling mainly caused the growth inhibition. The fitting model predictive analyses show, the critical concentration of Pb, which causes the germination rate and biomass fresh weight reducing 10%, is 195.18, 101.65 mg x L(-1).

Studies on accumulation of active ingredients, N, P and K in Anemarrhena asphodeloides / 中国中药杂志

<p><b>OBJECTIVE</b>To study the accumulation of active ingredients, the absorption and transformation of N, P and K in Anemarrhena asphodeloides and provide basis for determination of the harvest time and fertilizing.</p><p><b>METHOD</b>Samples were collected in different phrases and the weight of dry matter, the content of N, P and K of different organs and the content of sarsasapogenin were determined.</p><p><b>RESULT</b>Absorption of N, P and K started by the root and rhizoma after July. At the end of August, the N and K of the aerial part transfered largely into rhizome. The content of sarsasapogenin in rhizome was the highest in early spring.</p><p><b>CONCLUSION</b>Additional fertilizer is helpful to increase the yield in July of the second year after the transplantation. The quality is the best when harvest in early spring.</p>

Study on conditions of seed germination of Cistanche / 中国中药杂志

<p><b>OBJECTIVE</b>To study the effect of fluridone concentration, stimulating period, temperature and salt on the seed germination of three species of Cistanche.</p><p><b>METHOD</b>The seeds were cultured in Petri dish, and the germination percentage was counted.</p><p><b>RESULT</b>The highest germination percentage was observed in Cistanche tubulosa, C. deserticola, C. sala seeds pre-treated by 0.1 mg x L(-1) fluridone for 24-29 h. The optimal temperature for the seeds germination of three species of Cistanche was at 20-30 degrees C, and the seeds did not germinate at sub-or supraoptimal temperatures (5 and 35 degrees C). The salt tolerance of C. sala seeds was strong, and the critical value of NaCl concentration was 0.04 mol x L(-1). By contrast, C. tubulosa and C. deserticola seeds were more sensitive to the salt stress, the critical value of NaCl concentration was 0.02 mol x L(-1).</p><p><b>CONCLUSION</b>The optimal germination condition and the method of testing germination percentage of three species of Cistanche seeds are as follow: the seeds are pre-treated by 0.1 mg x L(-1) fluridone for 24 h and then cultured at 20-30 degrees C in salt solution which concentration is lower than 0.02 mol x L(-1).</p>

Studied of dry matter accumulation and echinacoside content of Cistanche tubulosa in Huabei plain / 中国中药杂志

<p><b>OBJECTIVE</b>To give some theory support of Cistanche tubulosa cultivation by searching dry matter accumulation and echinacoside content of C. tubulosa.</p><p><b>METHOD</b>Dry matter accumulation content of C. tubulosa culturing in Huabei plain was analysed in different growth season of C. tubulosa. Echinacoside content was determined by HPLC.</p><p><b>RESULT</b>Dry matter accumulation of C. tubulosa showed "S" variation. Dry matter accumulation increased fastest in September among growing seasons. Dry matter amount was 138.58 g after C. tubulosa grew a year. Dry matter amount decreased significantly along with inoculation time retarded. Echinacoside content was 30.59% when C. tubulosa grew in 5 months, decreased guadully after that, and 9.76% in annual.</p><p><b>CONCLUSION</b>Variation rule of dry matter accumulation and echinacoside content was found in C. tubulosa that grew one year in Huabei plain.</p>

Morphology of seed germination and parasitism in Cistanche tubulosa / 中国中药杂志

<p><b>OBJECTIVE</b>To understand the process of Cistanche tubulosa.</p><p><b>METHOD</b>The process of seed germination and parasitism was observed using stereomicroscope.</p><p><b>RESULT</b>Seedling of C. tubulosa sprouted after forty day without host root's contact in fields, a tube-like-organ formed and grew auger-type from host root, the tuber apex where touches host root swelled and formed haustorium. Haustorium intruded host root epidermis and vascular bundles, and released brown substances. Then, embryo bud with six or more young leaves formed, finally the swelled tuber-like-organ broken and seed coat shed. Due to the parasitism of C. tubulosa, the host root near stem site swelled, but the other part, shrunk and disappered gradually.</p><p><b>CONCLUSION</b>Seed of C. tubulosa could germinate indepently in fields. Tuber-like-organ formatin, haustorium formation and bud formation are key steps of C. tubulosa seedling development.</p>

Study on inoculation technology of Cistanche tubulosa in the field / 中国中药杂志

<p><b>OBJECTIVE</b>To increase inoculation rate of Cistanche tubulosa in the field by studying inoculation technologies.</p><p><b>METHOD</b>Root-tube inoculation methed was used on field experiments. Inoculation rate of C. tubulosa was compared to different size seeds and inoculation mediums and inoculation time.</p><p><b>RESULT AND CONCLUSION</b>May is suitable inoculation time. The inoculation rate of C. tubulosa is 92.5% while the seed width is more than 0.7 mm and coarse sand is selected during inoculation period.</p>

Effects of cultivation technique on yield and favonoid content of Chrysanthemum flower (Qiju) grown in Hebei / 中国中药杂志

<p><b>OBJECTIVE</b>To study the effect of cultivation techniques on the flower yield flavonoid content in Chrysanthemum flower grown in Hebei.</p><p><b>METHOD</b>Studied on flowers yield and three factors (transplanting date and plant density and fertilizer quantity) were examined in field experiment at 4 treatments levels.</p><p><b>RESULT AND CONCLUSION</b>The best results were obtained at following conditions: diammonium phosphate 300 kg x hm(-2) and potassium sulfate 150 kg x hm(-2) fertilized before transplanting, transplanting at the first ten days of May and the spacing 40 cm x 40 cm.</p>