RESUMO
To investigate the immune activation effect and mechanism of low molecular weight saccharides from Cistanche deserticola(LMSC) on mouse peritoneal macrophages, RAW264.7 cells. The RAW264.7 cells were divided into the normal control group, LPS positive control group, and LMSC treatment groups. The RAW264.7 cells were treated with various concentrations of LMSC from 3.91 to 62.5 g•L ⁻¹. The neutral red assay was employed to detect the phagocytic activity of macrophages. NO release was detected by using NO kit, and macrophage activation associated protein expression levels (TNF-α, IL-6, IKKβ, p-IKKβ, IκBα, p-IκBα, NF-κB, and p-NF-κB) were detected by Western blot. Results showed that LMSC had an activation effect on macrophages; it can significantly increase the release of NO in RAW264.7 cells and promote the expression of cytokines TNF-α and IL-6. Moreover, LMSC significantly increased the phosphorylation of IKKβ, IκBα, and NF-κB p65. Furthermore, mannitol's one of the main constituents in LMSC significantly enhanced the phagocytic activity of macrophages. These results showed that LMSC could activate macrophages by up-regulating the NF-κB signaling pathway, and mannitol may be one of the main active components in LMSC.
RESUMO
The clinical practice of bloodletting method on the face is introduced in this paper through 4 medical cases: post-operative periarthritis of the shoulder in lung cancer, dizziness and vertigo, protrusion of lumbar intervertebral disc and keratitis. It is believed that the change in facial luster, vein condition on the face, the site with the most obvious change in facial luster and local skin abnormality are commonly regarded as the reaction points or areas of facial disease. According to the reaction points or areas on the face and in association with the syndrome differentiation in Lingshu: Wuse (Miraculous Pivot : Five Colors), the acupoints are selected and stimulated with bloodletting method in the treatment of some difficult and complicated cases, and the good efficacy could be obtained. But, this therapeutic method needs a further research and deserves to be promoted in practice.