RESUMO
Anaplasma species are obligate intracellular pathogens that can cause tick-borne diseases in mammalian hosts. To date, very few studies of their occurrence in Korean native goats (Capra aegagrus hircus) have been reported. In the present study, we investigated Anaplasma infection of Korean native goats on Jeju Island, Republic of Korea, and performed phylogenetic analysis based on the 16S rRNA gene sequences. Our results showed that Anaplasma infection was found mostly in adult female goats. The phylogenetic tree revealed that the 7 sequences identified in Korean native goats could belong to Anaplasma sp. and were distinct from A. marginale, A. centrale, and A. ovis. The results indicated that the sequences identified to belong to Anaplasma were closely related to sequences isolated from goats in China and were clustered within the same group. To our knowledge, this is the first study to detect Anaplasma sp. infection in Korean native goats.
Assuntos
Animais , Feminino , Masculino , Anaplasma/classificação , Anaplasmose/microbiologia , Doenças das Cabras/microbiologia , Cabras , Ilhas , Dados de Sequência Molecular , Filogenia , República da CoreiaRESUMO
The objective of this study was to investigate the prevalence of tick-borne pathogens in the Korean water deer (Hydropotes inermis argyropus). Pathogens were identified using PCR which included Anaplasma, Ehrlichia, Rickettsia, and Theileria. Rickettsia was not detected, whereas Anaplasma, Ehrlichia, and Theileria infections were detected in 4, 2, and 8 animals, respectively. The most prevalent pathogen was Theileria. Of the 8 Theileria-positive animals, 2 were mixed-infected with 3 pathogens (Anaplasma, Ehrlichia, and Theileria) and another 2 animals showed mixed-infection with 2 pathogens (Anaplasma and Theileria). Sequencing analysis was used to verify the PCR results. The pathogens found in this study were identified as Anaplasma phagocytophilum, Ehrlichia canis, and Theileria sp. To the best of our knowledge, this is the first report identifying these 3 pathogens in the Korean water deer. Our results suggest that the Korean water deer may serve as a major reservoir for these tick-borne pathogens, leading to spread of tick-borne diseases to domestic animals, livestock, and humans. Further studies are needed to investigate their roles in this respect.
Assuntos
Animais , Anaplasma/isolamento & purificação , Infecções Bacterianas/epidemiologia , Análise por Conglomerados , Coinfecção/epidemiologia , DNA Bacteriano/química , DNA Ribossômico/química , Ehrlichia/isolamento & purificação , Coreia (Geográfico)/epidemiologia , Dados de Sequência Molecular , Filogenia , Prevalência , RNA Ribossômico 16S/genética , Rickettsia/isolamento & purificação , Ruminantes/microbiologia , Análise de Sequência de DNA , Theileria/isolamento & purificaçãoRESUMO
Primary pelvic non-Hodgkin's lymphoma is a rare malignancy that has been reported in the retroperitoneum, ovary, uterine corpus, uterine cervix, vagina and vulva. Although primary pelvic lymphoma is rare, this may be encountered more frequently as the incidence of extranodal non-Hodgkin's lymphoma increases. We experienced three cases of primary non-Hodgkin's lymphoma of the pelvis. Two cases are primary non-Hodgkin's lymphoma of the ovary and one is primary non-Hodgkin's lymphoma of the retroperitoneum. We report these cases with a brief review of literature.