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Acta Pharmaceutica Sinica ; (12): 102-108, 2011.
Artigo em Chinês | WPRIM | ID: wpr-353324

RESUMO

Polyamidoamine-polyethylene glycol (PAMAM-PEG) copolymers were synthesized using IPDI as coupling reagent by two-step method. The copolymers were characterized by IR spectrum and 1H NMR spectrum, and the PEG conjugating ratios of the copolymers were calculated equal to 10% and 30% separately. MTT assay indicated that after PEGylation a lower cytotoxicity of the copolymers could be found, and with increasing PEG conjugating ratio the cytotoxicity decreased obviously. Agarose gel retardation assay demonstrated that PAMAM-PEG copolymers could be combined with DNA and PAMAM-PEG/DNA complexes were prepared by self-assembly. DLS measurement showed that when N/P > or = 50, the particle size of copolymer/ gene complexes was in a range of 150-200 nm, and the zeta potential was in a range of 10-25 mV. In vitro gene transfection illustrated that when N/P < or = 50, the gene transfection efficiency of PAMAM-PEG copolymers was a little less than that of PAMAM-G5, but the transfection efficiency can be raised by increasing N/P ratio or transfection time. Considering both cytotoxicity and transfection efficiency aspects PAMAM-PEG-13 was more effect than PAMAM-PEG-39 in PEGylation.


Assuntos
Humanos , Carcinoma Hepatocelular , Patologia , Linhagem Celular Tumoral , Sobrevivência Celular , DNA , Química , Farmacologia , Dendrímeros , Farmacologia , Técnicas de Transferência de Genes , Vetores Genéticos , Isocianatos , Química , Neoplasias Hepáticas , Patologia , Tamanho da Partícula , Poliaminas , Química , Polietilenoglicóis , Química , Farmacologia , Transfecção
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