Correlation of EBV Infection with Expression of TNF-α-Inducing Protein 3 Gene and A20 Protein in Hodgkin's Lmphoma / 中国实验血液学杂志

OBJECTIVE@#To analyze the correlation of EBV infection with expression of TNF-α-inducing protein 3 gene and A20 protein in Hodgkin lmphoma.@*METHODS@#The clinical data and pathological specimens of 65 cases of Hodgkin's lymphoma in our hospital were analyzed retrospectively, and the tissue chips were made for the rich area of the tumor cells. The latent membrane protein 1 encoded by EBV was measured by immunohistochemical staining, and the RNA encoded by EBV was measured by in situ hybridization to analyze the infection state. The gene expression of tumor necrosis factor.α-induced protein 3 was detected by fluorescence in situ hybridization, and the expression of A20 protein encoded by EBV was detected by immunohistochemical staining. The obtained data were processed by SPSS 23.0 version statistical software.@*RESULTS@#The positive rate of latent membrane protein 1 was 26.15% (17/65), the positive rate of EBV encoded RNA was 26.15% (17/65), and the coincidence rate was 100.00%. In 65 patients, A20 protein expression was lost in 18 cases (27.69%), and 14 cases (21.54%) showed homozygous or heterozygous deletion of tumor necrosis factorα protein 3 gene. Only 1 case showed A20 loss combined with homozygous deletion of TNFα inducible protein 3. Correlation analysis showed that EBV infection did not significantly relate with expression loss of A20 protein and the gene deletion of TNF-α inducing protein 3 (P>0.05).@*CONCLUSION@#The expression loss of A20 protein and gene detection of TNFα inducing protein 3 are found in both EBV negative and positive patients with Hodgkin's lymphoma, however the results of immunohistochemical staining and fluorescence in situ hybridization are not complete consistant, the reason may closely relate with the technical factors.

Effect of Lymphocyte/Monocyte Ratio on Clinical Features and Prognosis of Patients with Primary Gastrointestinal Diffuse Large B Cell Lymphoma / 中国实验血液学杂志

<p><b>OBJECTIVE</b>To analyze the effect of lymphocyte/monocyte ratio(LMR) on clinical features and prognosis of patients with primary gastrointestinal diffuse large B cell lymphoma(PGI-DLBCL).</p><p><b>METHODS</b>The clinical data of 38 PGI-DLBCL patients with complete follow-up data in our hospital were analyzed retrospectively. The absolute lymphocyte count(ALC), absolute monocyte count(AMC) and LMR were counted by automating complete blood cell count statistics in newly diagnosed patients, and then the LMR cut off value was obtained by ROC curve. All patients were divided into ≤3.9 group and >3.9 group according to cut off value.</p><p><b>RESULTS</b>Out of 38 patients 21 male and 17 female with a median age 55 old years (29-73 years), 7 cases died, the clinical B symptom occurred in 7 cases (18.4%); the pathologic type of 13 cases (34.2%) belonged to germinal center B-cell like, the primary gastral and intestinal DLBCL were observed in 18 and 20 cases respectively. The chisquare test showed that the LMR associated with of clinical stage and tumor size of PGI-DLBCL. The median survival time was 44 months (7-100 months), and 5-year overrall survival(OS) rate was 78.3% for 38 PGI-DLBCL cases. The univariate analysis showed that age (P=0.021), stage (P=0.012), IPI score (P=0.001), LDH level (P<0.001), tumor size (P=0.037) and LMR (P=0.026) all associate with the 5 years OS rate(%), and the difference was between them statistically significant, but the multivariate analysis showed that only clinical staging is independent risk factors for the OS.</p><p><b>CONCLUSION</b>LMR shows an important effect on clinical features and prognosis of PGI-DLBCL.</p>

Treatment analysis of 26 patients with breast ductal carcinoma in situ / 中华肿瘤杂志

<p><b>OBJECTIVE</b>To study the appropriate surgical treatment for breast ductal carcinoma in situ (DCIS).</p><p><b>METHODS</b>Twenty-six such patients treated between 1992 and 2001 were retrospectively analyzed. Among them, 3 patients were treated by simple mastectomy, 23 patients by mastectomy and axillary lymph node dissection, 8 patients by chemotherapy and one patient by radiotherapy after operation. Median follow-up was 42 m (rang 12 - 112 m).</p><p><b>RESULTS</b>Except 3 of these 26 patients lost in follow-up and 1 patient died from diabetes mellitus, all the other 22 patients survived over 5 years. All lymph nodes dissected from 23 patients were negative. After surgery, 3 patients developed lymph edema of the arm.</p><p><b>CONCLUSION</b>DCIS, lacking the potential of metastasis, is not invasive. Conservative breast surgery without lymph node dissection is feasible for most DCIS patients.</p>