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1.
Acta Physiologica Sinica ; (6): 267-271, 2011.
Artigo em Chinês | WPRIM | ID: wpr-335991

RESUMO

The present study aims to investigate the effects of soluble endoglin (sEng) on invasive ability of cultured cytotrophoblasts of first trimester of pregnancy. Cytotrophoblasts of normal 6 to 8-week pregnancy were cultured by trypsin digestion method, and were incubated with cell culture medium without (control group) and with 10 μg/L sEng (sEng group), respectively for 24 h. The invasive ability was determined by transwell invasion assay, and expressions of MMP-2, MMP-9 mRNA and protein were detected by reverse transcription-polymerase chain reaction (RT-PCR) and Western blot, respectively. The results showed that the invasive ability of cytotrophoblasts in sEng group was lower than that in control group (P < 0.05). Compared with control group, the expressions of MMP-2 and MMP-9 mRNA and protein of cytotrophoblasts were significantly lower (P < 0.05). In conclusion, sEng may participate in the genesis of preeclampsia by affecting the invasive ability of cytotrophoblasts through regulation of the expression of MMP-2 and MMP-9.


Assuntos
Feminino , Humanos , Gravidez , Antígenos CD , Farmacologia , Movimento Celular , Fisiologia , Células Cultivadas , Endoglina , Metaloproteinase 2 da Matriz , Genética , Metabolismo , Metaloproteinase 9 da Matriz , Genética , Metabolismo , Placentação , Fisiologia , Pré-Eclâmpsia , Primeiro Trimestre da Gravidez , RNA Mensageiro , Genética , Metabolismo , Receptores de Superfície Celular , Trofoblastos , Biologia Celular
2.
China Journal of Chinese Materia Medica ; (24): 1777-1779, 2007.
Artigo em Chinês | WPRIM | ID: wpr-287896

RESUMO

<p><b>OBJECTIVE</b>To study the effects of volatie oil of Schizonepeta tenuifolia Briq herb and Saposhnikovia divaricata Schischke root (OSS) on proinflammatory cytokine expression and regulation in rats.</p><p><b>METHOD</b>OA and LPS were injected intravenously to rats to develop acute lung injury (ALI). The rats were treated with OSS (45.19 microL kg(-1)). The pathological sections of lung tissue were prepared and observed in acute lung injury rats. The expression of nuclear factor-kappa B p65 (NF-kappaB p65), intercellar adhesion molecule CD54, and NF-kappaB p65 mRNA were determined in lung cells.</p><p><b>RESULT</b>volatie oil of Schizonepeta tenuifolia Briq herb and Saposhnikovia divaricata Schischke root significantly inhibited the expression of CD54, the activation of NF-kappaB p65, and the transcription of NF-kappaB p65 mRNA.</p><p><b>CONCLUSION</b>OSS can reduce the expression of CD54 and NF-kappaB p65 protein synthesis, which may be its anti-inflammatory molecular mechanisms.</p>


Assuntos
Animais , Masculino , Ratos , Anti-Inflamatórios , Farmacologia , Apiaceae , Química , Combinação de Medicamentos , Regulação da Expressão Gênica , Imuno-Histoquímica , Molécula 1 de Adesão Intercelular , Lamiaceae , Química , Lipopolissacarídeos , Óleos Voláteis , Farmacologia , Ácido Oleico , Óleos de Plantas , Farmacologia , Plantas Medicinais , Química , RNA Mensageiro , Genética , Distribuição Aleatória , Ratos Wistar , Síndrome do Desconforto Respiratório , Metabolismo , Fator de Transcrição RelA , Genética
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