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@#Objective To develop a large-scale culture process for rabies virus(RABV)in 150 L bioreactor,and lay a foundation for the further development of a larger-scale and high-density microcarrier reactor process.Methods Vero cells and RABV strain CTN-1V were cultured in 30 L(model:C30-2)and 150 L(model:VESSEL FERMENTER 300L)bioreactors by perfused culture with 20 g/L Cytodex-1 microcarrier and DO 20%-60%,at culture temperature 36-38 ℃ and pH 7.0-7.4.During the culture process,the cell density and virus titer were measured.The virus culture media was harvested for consecutive 13 d and detected for the sterility,mycoplasma,and the residues of antigen,host cell protein(HCP),bovine serum albumin(BSA)and DNA.Results The density of cultured cells in 30 L and 150 L bioreactors all reached above 1.2 ×10~7cell/mL.There was no significant difference in cell density at different time points during the culture(t = 0.225-2.173,P = 0.096-0.833).The highest virus titer(8.5 lgLD_(50)/mL)was found in the both bioreactors 6 d after infection with no significant difference(t = 1.000,P = 0.374).The residues of antigen,HCP,BSA and DNA in the virus suspension from the two bioreactors were basically the same.Conclusion 150 L bioreactor can be used for the large-scale culture of RABV,and the harvested virus conformed to the relevant standards in Chinese Pharmacopoeia(Volume Ⅲ,2020 edition).
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Vascular endothelium play an essential role in regulating endothelial functions by maintaining normal vascular tone, modulating hemostasis, and preventing thromobogenesis. The aim of present study was to reveal ultrastructure features of coronary artery endothelium in Bactrian Camels by means of scanning electron microscope (SEM) technology. The results showed that the endothelial cells distribute along the direction of blood flow. Their morphology and protuberant degree towards luminal surface vary for different parts of the coronary artery. The luminal surface of a.c oronaria sinistra and a. coronaria dextra was covered by impaired and intact endothelium, respectively. Platelets and leukocytes sticking to the endothelium were found.
Las células endoteliales de los vasos sanguíneos juegan un rol esencial en la regulación de las funciones endoteliales manteniendo el tono vascular, modulando la homeostasis y previniendo la trombogénesis. En la investigación se utilizó un microscopio electrónico de barrido, revelando las características estructurales de las células endoteliales de los vasos coronarios de los camellos bactrianos. Los resultados indican que las células endoteliales estaban distribuidas a lo largo de la dirección de flujo. En distintas partes de la arteria coronaria existen diferencias en el grado de proyección de la morfología y de la superficie de la cavidad de dichas células. La superficie luminal de la arteria coronaria izquierda y la arteria coronaria derecha estaban cubiertas por endotelio deteriorado e intacto, respectivamente. Se observó adhesión de leucocitos y plaquetas en la superficie de las células endoteliales.