RESUMO
AIM@#To study the bufadienolides in the Chinese traditional drug "Ch'an Su" and their cytotoxic activity.@*METHOD@#Various chromatographic techniques were used to isolate the constituents, and their structures were elucidated through physical and spectroscopic data.@*RESULTS@#Twenty compounds were isolated, and eighteen were evaluated in vitro for their cytotoxic activity against A-549 and K-562 cells.@*CONCLUSION@#Compound 1 (bufalin 3β-acrylic ester) was a new bufadienolide and exhibited the most potent activity against the two tumor cell lines with IC50 values of 7.16 and 6.83 nmol · L(-1). The relationships between structure and activity are discussed.
Assuntos
Humanos , Venenos de Anfíbios , Química , Farmacologia , Usos Terapêuticos , Antineoplásicos , Química , Farmacologia , Usos Terapêuticos , Produtos Biológicos , Química , Farmacologia , Usos Terapêuticos , Bufanolídeos , Química , Farmacologia , Usos Terapêuticos , Concentração Inibidora 50 , Células K562 , Medicina Tradicional Chinesa , Estrutura Molecular , Neoplasias , Tratamento Farmacológico , Relação Estrutura-AtividadeRESUMO
A new hasubanan alkaloid, hernsubanine E (1), as well as two known compounds p-hydroxybenzaldehyde (2) and (-)-syringaresinol (3) have been isolated from the whole plants of Stephania hernandifolia by various column chromatographic methods. Their structures were identified by physicochemical properties and spectral analyses. Compounds 2 and 3 were isolated from the genus of Stephania for the first time.
Assuntos
Alcaloides , Química , Compostos Heterocíclicos de 4 ou mais Anéis , Química , Stephania , QuímicaRESUMO
<p><b>OBJECTIVE</b>To study the chemical constituents in the spikes of Schizonepeta tenuifolia.</p><p><b>METHOD</b>Compounds were isolated and purified with silica gel, ODS and Sephadex LH-20 gel column chromatography, and their structures were determined by using spectroscopic analysis including MS and NMR.</p><p><b>RESULT</b>Nine compounds were isolated and identified as 5, 7-dihydroxy-6, 4'-dimethoxyflavone (1), 5, 7-dihydroxy-6, 3', 4'-trimethoxyflavone (2), ursolic acid (3), 3-hydroxy-4(8)-ene-p-menthane-3(9)-lactone (4), 5, 7, 4'-trihydroxyflavone (5), 5, 4'-dihydroxy-7-methoxyflavone (6), hesperidin (7), luteolin (8) and daucesterol (9).</p><p><b>CONCLUSION</b>Compounds 1, 2, 6 were first obtained from the spikes of S. tenuifolia.</p>
Assuntos
Flavonas , Química , Topos Floridos , Química , Lamiaceae , Química , Plantas Medicinais , QuímicaRESUMO
<p><b>OBJECTIVE</b>To study the chemical constituents of Mentha haplocalyx.</p><p><b>METHOD</b>The chemical constituents were isolated and purified with column chromatography and the structures were elucidated by spectral analysis.</p><p><b>RESULT</b>Nine compounds were obtained and identified as emodin (I), chrysophanol (II), physcione (II), benzoic acid (IV), trans-cinnamic acid (V), beta-sitosterol (VI), aloe-emodin (VII), ursolic acid (VIII) and daucosterol (IX).</p><p><b>CONCLUSION</b>Compounds I, II, III, V, VII were first isolated from M. haplocalyx.</p>